sulfo-Cyanine7.5 antibody labeling kit

Cat. # Quantity Price Lead time
6321-1rxn
1 rxn
$69 1 days
6321-10rxn
10 rxn
$450 1 days
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A ready-to-use kit for labeling antibodies with sulfo-Cyanine7.5 dye. This kit contains all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean-up is achieved using enclosed gel filtration spin columns.

Precise quantification of the dye guarantees the reproducible outcome of the labeling and number of fluorophores per antibody molecule.

Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands-on time). The kit tolerates common additives such as Tris, glycerol, and sodium azide.

Absorption and emission spectra of sulfo-Cyanine7.5

Absorption and emission spectra of sulfo-Cyanine7.5

Ready-to-use kit manual

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General properties

Storage conditions: Store at temperature between +4 °C and +20 °C. Do not freeze!
MSDS: Download

Spectral properties

Excitation/absorption maximum, nm: 778
ε, L⋅mol−1⋅cm−1: 222000
Emission maximum, nm: 797
CF260: 0.09
CF280: 0.09

Kit

Storage time in months: 12

References

  1. Melentiev, P.N.; Son, L.V.; Kudryavtsev, D.S.; Kasheverov, I.E.; Tsetlin, V.I.; Esenaliev, R.O.; Balykin, V.I. Ultrafast, Ultrasensitive Detection and Imaging of Single Cardiac Troponin-T Molecules. ACS Sensors, 2020, 5(11), 3576–3583. doi: 10.1021/acssensors.0c01790
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