This is a kit for the labeling of antibodies and proteins of similar size with sulfo-Cyanine7.5 fluorophore. The dye is attached by NHS ester chemistry, and then the excess of the fluorophore is removed by means of gel filtration using enclosed spin columns. The protocol requires only around 40 minutes of time, and provides desalted antibody in PBS buffer containing optimal number of fluorophores per Ab. The kit contains components for 10 reactions, up to 100 ug of antibody per reaction.
Absorption and emission spectra of sulfo-Cyanine7.5