sulfo-Cyanine5 antibody labeling kit
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-1rxn |
1 rxn
|
$69
|
1 days | |
| 3321-10rxn |
10 rxn
|
$397
|
1 days |
A ready-to-use kit for labeling antibodies with sulfo-Cyanine5 dye. This kit contains all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean-up is achieved using enclosed gel filtration spin columns.
Precise quantification of the dye guarantees the reproducible outcome of the labeling and number of fluorophores per antibody molecule.
Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands-on time). The kit tolerates common additives such as Tris, glycerol, and sodium azide.
Product in action
Human blood smear stained with green fluorescent nuclear stain LUCS13 and antibodies to neutrophil myeloperoxidase
Human blood smear stained with blue fluorescent nuclear stain DAPI and antibodies to neutrophil myeloperoxidase
Absorption and emission spectra of sulfo-Cyanine5
Ready-to-use kit manual
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Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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General properties
| Storage conditions: | Store at temperature between 4°C and 20°C. Do not freeze! Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| Emission maximum, nm: | 662 |
Kit
| Storage time in months: | 12 |
References
- Hu, K.; Harman, A.; Baharlou, H. Imaging Mass Cytometry for In Situ Immune Profiling. Flow Cytometry Protocols, 2024, 2779, 407-423. doi: 10.1007/978-1-0716-3738-8_19
- Rana, H.; Truong, N.R.; Johnson, B.; Baharlou, H.; Herbert, J.J.; Kandasamy, S.; Goddard, R.; Cohen, R.C.; Wines, M.; Nasr, N.; Harman, A.N.; Bertram, K.M.; Sandgren, K.J.; Cunningham, A.L. Herpes simplex virus spreads rapidly in human foreskin, partly driven by chemokine-induced redistribution of Nectin-1 on keratinocytes. PLOS Pathogens, 2024, 20(6), e1012267. doi: 10.1371/journal.ppat.1012267
- Kirikovich, S.S.; Levites, E.V.; Proskurina, A.S.; Ritter, G.S.; Peltek, S.E.; Vasilieva, A.R.; Ruzanova, V.S.; Dolgova, E.V.; Oshihmina, S.G.; Sysoev, A.V.; Koleno, D.I.; Danilenko, E.D.; Taranov, O.S.; Ostanin, A.A.; Chernykh, E.R.; Kolchanov, N.A.; Bogachev, S.S. The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF. International Journal of Molecular Sciences, 2023, 24(24), 17396. doi: 10.3390/ijms242417396
- Rhodes, J. W.; Botting, R. A.; Bertram, K. M.; Vine, E. E.; Rana, H.; Baharlou, H.; Vegh, P.; O’Neil, T. R.; Ashhurst, A. S.; Fletcher, J.; Parnell, G. P.; Graham, J. D.; Nasr, N.; Lim, J. J. K.; Barnouti, L.; Haertsch, P.; Gosselink, M. P.; Di Re, A.; Reza, F.; Ctercteko, G.; Jenkins, G. J.; Brooks, A. J.; Patrick, E.; Byrne, S. N.; Hunter, E.; Haniffa, M. A.; Cunningham, A. L.; Harman, A. N. Human Anogenital Monocyte-Derived Dendritic Cells and Langerin+cDC2 Are Major HIV Target Cells. Nat Commun, 2021, 12(1), 2147. doi: 10.1038/s41467-021-22375-x
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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