sulfo-Cyanine5 antibody labeling kit

sulfo-Cyanine5 NHS esters Protein labeling
Cat. # Quantity Price Lead time
3321-1rxn
1 rxn
$69 21 days
3321-10rxn
10 rxn
$397 21 days
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A ready-to-use kit for labeling antibodies with sulfo-Cyanine5 dye. This kit contains all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean-up is achieved using enclosed gel filtration spin columns.

Precise quantification of the dye guarantees the reproducible outcome of the labeling and number of fluorophores per antibody molecule.

Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands-on time). The kit tolerates common additives such as Tris, glycerol, and sodium azide.

sulfo-Cyanine5 antibody labeling kit, 3321-10rxn
sulfo-Cyanine5 antibody labeling kit, 3321-10rxn

Product in action

Human blood smear stained with green fluorescent nuclear stain LUCS13 and antibodies to neutrophil myeloperoxidase

Human blood smear stained with blue fluorescent nuclear stain DAPI and antibodies to neutrophil myeloperoxidase

Absorption and emission spectra of sulfo-Cyanine5

Absorption and emission spectra of sulfo-Cyanine5

Ready-to-use kit manual

Calculator

General properties

Storage conditions: Store at temperature between +4 °C and +20 °C. Do not freeze!
MSDS: Download

Kit

Storage time in months: 12

References

  1. Hu, K.; Harman, A.; Baharlou, H. Imaging Mass Cytometry for In Situ Immune Profiling. Flow Cytometry Protocols, 2024, 2779, 407-423. doi: 10.1007/978-1-0716-3738-8_19
  2. Kirikovich, S.S.; Levites, E.V.; Proskurina, A.S.; Ritter, G.S.; Peltek, S.E.; Vasilieva, A.R.; Ruzanova, V.S.; Dolgova, E.V.; Oshihmina, S.G.; Sysoev, A.V.; Koleno, D.I.; Danilenko, E.D.; Taranov, O.S.; Ostanin, A.A.; Chernykh, E.R.; Kolchanov, N.A.; Bogachev, S.S. The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF. International Journal of Molecular Sciences, 2023, 24(24), 17396. doi: 10.3390/ijms242417396
  3. Rhodes, J. W.; Botting, R. A.; Bertram, K. M.; Vine, E. E.; Rana, H.; Baharlou, H.; Vegh, P.; O’Neil, T. R.; Ashhurst, A. S.; Fletcher, J.; Parnell, G. P.; Graham, J. D.; Nasr, N.; Lim, J. J. K.; Barnouti, L.; Haertsch, P.; Gosselink, M. P.; Di Re, A.; Reza, F.; Ctercteko, G.; Jenkins, G. J.; Brooks, A. J.; Patrick, E.; Byrne, S. N.; Hunter, E.; Haniffa, M. A.; Cunningham, A. L.; Harman, A. N. Human Anogenital Monocyte-Derived Dendritic Cells and Langerin+cDC2 Are Major HIV Target Cells. Nat Commun, 2021, 12(1), 2147. doi: 10.1038/s41467-021-22375-x
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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