DiA, lipophilic tracer

Cat. # Quantity Price Lead time
2935-5mg 5 mg –   in stock
2935-50mg 50 mg $159 in stock
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DiA is a dialkylaminostyryl dye that fluoresces when inserted into membranes or diluted into organic solvents. DiA’s excitation max is 492 nm, and emission max is 607 nm. Due to its broad emission spectrum, DiA can be detected in the green, orange, and red channels depending on the filter used.

DiA is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiA uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.

DiA can be used as a second-color neuronal tracer in conjunction with other neuronal tracers, for example, DiI.

Absorption and emission spectra of DiA

Absorption and emission spectra of DiA

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General properties

Appearance: red-brown solid
Molecular weight: 787.06
CAS number: 114041-00-8
Molecular formula: C46H79IN2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 50000
Emission maximum, nm: 607
Fluorescence quantum yield: 0.87
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