Cyanine3B DBCO
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
193F0 | 1 mg | $369 | in stock | |
293F0 | 5 mg | $727 | in stock | |
493F0 | 25 mg | $1950 | in stock |
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
Cyanine3B is a yellow-emitting cyanine dye that is an improved version of the Cyanine3 fluorophore with significantly higher fluorescence quantum yield and photostability. Due to the fixed conformation, Cyanine3B has the highest emission quantum yield compared to other dyes of this wavelength.
This is a sulfonated dye and can be used for efficient labeling in water media.
Absorbance and emission spectra of Cyanine3B
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A diethylene glycol (PEG2) linker with azide and amino groups.sulfo-Cyanine3 DBCO
sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water-soluble fluorophore sulfo-Cyanine3 for copper-free click chemistry.General properties
Appearance: | dark red powder |
Molecular weight: | 861.08 |
Molecular formula: | C52H52N4O6S |
Solubility: | soluble in methanol, ethanol, DMF, DMSO |
Quality control: | NMR 1H and HPLC-MS (90+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 559 |
ε, L⋅mol−1⋅cm−1: | 121000 |
Emission maximum, nm: | 571 |
Fluorescence quantum yield: | 0.68 |
CF260: | 0.044 |
CF280: | 0.077 |