AF 555 DBCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1K8F0 | 1 mg |
$140.00
|
in stock | |
| 2K8F0 | 5 mg |
$450.00
|
in stock | |
| 4K8F0 | 25 mg |
$1460.00
|
in stock | |
| 5K8F0 | 50 mg |
$1890.00
|
in stock | |
| 6K8F0 | 100 mg |
$2990.00
|
in stock |
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF 555 is a hydrophilic fluorophore with high fluorescence quantum yield and high photostability, an alternative to tetramethylrhodamine (TAMRA, TMR) or Cyanine3 dyes.
AF 555 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Absorption and emission spectra of AF 555
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General properties
| Appearance: | purple powder |
| Molecular weight: | 1279.71 |
| Molecular formula: | C56H65K3N4O15S4 |
| Solubility: | water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 152000 |
| Emission maximum, nm: | 566 |
| Fluorescence quantum yield: | 0.14 |
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