Cyanine3 carboxylic acid
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11090
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Free Cyanine3 carboxylic acid (Cy3® carboxylic acid analog), non-activated dye. Non-sulfonated reagent, with good solubility in organic solvents, and limited aquous solubility. The dye can be used as a non-reactive fluorophore, for control experiments, and for calibration.
For coupling with amines, and labeling, consider using Cyanine3 NHS ester , or water-soluble sulfo-Cyanine3 NHS ester .
Cyanine3 absorbance and emission spectra
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Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.
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General properties
Appearance:
red powder
Molecular weight:
493.08
CAS number:
1361402-15-4 (inner salt), 1032678-01-5 (chloride), 1251915-29-3 (iodide)
Molecular formula:
C30 H37 ClN2 O2
Solubility:
soluble in organic solvents (DMF, DMSO, dichloromethane), poorly soluble in water (1.8 g/L = 4.0 mM)
Quality control:
NMR 1 H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Product specifications
Spectral properties
Excitation/absorption maximum, nm:
555
ε, L⋅mol−1 ⋅cm−1 :
150000
Emission maximum, nm:
570
Fluorescence quantum yield:
0.31
CF260 :
0.04
CF280 :
0.09
Product citations
Nam, J.; Lim, H.-K.; Kim, N.H.; Park, J.K.; Kang, E.S.; Kim, Y.-T.; Heo, C.; Lee, O.-S.; Kim, S.-G.; Yun, W.S.; Suh, M.; Kim, Y.H. Supramolecular Peptide Hydrogel-Based Soft Neural Interface Augments Brain Signals through Three-Dimensional Electrical Network. ACS Nano , 2020 , 14(1), 664–675. doi: 10.1021/acsnano.9b07396
Deshmukh, R.; Biehs, S.-A.; Khwaja, E.; Galfsky, T.; Agarwal, G.S.; Menon, V.M. Long-range resonant energy transfer using optical topological transitions in metamaterials. ACS Photonics , 2018 , 5(7), 2737–2741. doi: 10.1021/acsphotonics.8b00484
Mattila, J.T.; Beaino, W.; Maiello, P.; Coleman, M.T.; White, A.G.; Scanga, C.A.; Flynn, J.A.; Anderson, C.J. Positron Emission Tomography Imaging of Macaques with Tuberculosis Identifies Temporal Changes in Granuloma Glucose Metabolism and Integrin α4β1-Expressing Immune Cells. Journal of immunology , 2017 , 199(2), 806–815. doi: 10.4049/jimmunol.1700231
Soni, K.S.; Lei, F.; Desale, S.S.; Marky, L.A.; Cohen, S.M.; Bronich, T.K. Tuning polypeptide-based micellar carrier for efficient combination therapy of ErbB2-positive breast cancer. Journal of Controlled Release , 2017 , 264, 276–287. doi: 10.1016/j.jconrel.2017.08.038
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