Cyanine3 carboxylic acid

Cyanine3 Carboxylic acids
Cat. # Quantity Price Lead time
11090 1 mg $110.00 in stock
21090 5 mg $210.00 in stock
41090 25 mg $410.00 in stock
51090 50 mg $695.00 in stock
61090 100 mg $1190.00 in stock
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Free Cyanine3 carboxylic acid (Cy3® carboxylic acid analog), non-activated dye. Non-sulfonated reagent, with good solubility in organic solvents, and limited aquous solubility. The dye can be used as a non-reactive fluorophore, for control experiments, and for calibration.

For coupling with amines, and labeling, consider using Cyanine3 NHS ester, or water-soluble sulfo-Cyanine3 NHS ester.

Cy3 free carboxylic acid structure
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Cyanine3 absorbance and emission spectra

Cyanine3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 493.08
CAS number: 1144107-76-5, 1361402-15-4, 1251915-29-3 [I-]
Molecular formula: C30H37ClN2O2
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Soni, K.S.; Lei, F.; Desale, S.S.; Marky, L.A.; Cohen, S.M.; Bronich, T.K. Tuning polypeptide-based micellar carrier for efficient combination therapy of ErbB2-positive breast cancer. Journal of Controlled Release, 2017, 264, 276–287. doi: 10.1016/j.jconrel.2017.08.038
  2. Melle, A.; Balaceanu, A.; Kather, M.; Wu, Y.; Gau, E.; Sun, W.; Huang, X.; Shi, X.; Karperien, M.; Pich, A. Stimuli-Responsive Poly(N}-vinylcaprolactam-co-2-methoxyethyl acrylate) Core-Shell Microgels: Facile Synthesis, Modulation of Surface Properties and Controlled Internalisation into Cells. Journal of Materials Chemistry B, 2016, 4(30), 5127–5137. doi: 10.1039/c6tb01196a
  3. Kwok, S.J.J.; Choi, M.; Bhayana, B.; Zhang, X.; Ran, C.; Yun, S.-H. Two-photon excited photoconversion of cyanine-based dyes. Scientific Reports, 2016, 6, 23866. doi: 10.1038/srep23866
  4. Xue, M.; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085–3093. doi: 10.1021/jacs.5b12187
Cy® is a trademark of GE Healthcare.
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