AF 488 carboxylic acid

Cat. # Quantity Price Lead time
11890 1 mg $125 in stock
21890 5 mg $325 in stock
41890 25 mg $950 in stock
51890 50 mg $1625 in stock
61890 100 mg $2090 in stock
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AF 488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF 488 can be used with DAPI and is well suited to multiplex assay. AF 488 has high quantum yield and stable fluorescence within the pH range from 4 to 10.

AF 488 carboxylic acid is a non-reactive AF 488 form that can be used as a reference standard in experiments where AF 488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF 488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: orange crystals
Molecular weight: 648.75
Molecular formula: C21H11K3N2O11S2
IUPAC name: 4-(6-amino-3-iminio-4,5-disulfonato-3H-xanthen-9-yl)isophthalate
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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