DAF-FM diacetate (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| SA100 | 50 ug |
$20.99
|
in stock | |
| 1A100 | 1 mg |
$349.90
|
in stock | |
| 2A100 | 5 mg |
$1290.00
|
in stock |
DAF-FM diacetate (DAF-FM DA) is a cell-permeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM DA passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases and converted to cell-impermeant form — DAF-FM.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM DA should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.
Fluorescence emission spectra of DAF-FM, free and in solution containing nitric oxide (NO)
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General properties
| Appearance: | beige solid |
| Molecular weight: | 496.42 |
| CAS number: | 254109-22-3 |
| Molecular formula: | C25H18F2N2O7 |
| Solubility: | DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
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