AF 568 TCO

Cat. # Quantity Price Lead time
148H0 1 mg
$318.00
in stock
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AF 568 TCO is a bright, photostable dye bearing a trans-cyclooctene (TCO) handle for rapid bioorthogonal labeling. It combines the excellent photophysical properties of the AF 568 fluorophore (orange—red emission, high quantum yield, and good photostability) with the fast and selective reactivity of the trans-cyclooctene group.

The TCO moiety undergoes ultrafast strain-promoted IEDDA ligation with tetrazine-functionalized targets, enabling rapid conjugation at low concentrations without copper catalysts and with minimal impact on biological systems.

The dye is well suited for fluorescence microscopy, multicolor imaging, and tracking of tetrazine-tagged proteins, nucleic acids, or small molecules, providing a bright signal with low background in biological samples.

Absorption and emission spectra of AF 568

Absorption and emission spectra of AF 568

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General properties

Appearance: deep-violet crystals
Molecular weight: 1147.51
Molecular formula: C60H86N6O12S2
Solubility: DMSO, DMF, water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 572
ε, L⋅mol−1⋅cm−1: 94238
Emission maximum, nm: 598
Fluorescence quantum yield: 0.912
CF260: 0.4
CF280: 0.32
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