QuDye dsDNA BR Assay Kit

Cat. # Quantity Price Lead time
A9102
10 assays
–   21 days
19102
100 assays
$105 $67 1 days
1Z102
/ includes tubes
100 assays
$125 $79 1 days
59102
500 assays
$290 $147 1 days
69102
/ includes tubes
500 assays
$389 $176 1 days
89102
1000 assays
$499 1 days
79102
/ aliquoted standard
1000 assays
$499 1 days
Found better price? Let us know and we will propose the way forward!

This kit is intended for quantification of double-stranded DNA in the broad range with fluorometer (any version can be used). The kit includes QuDye dsDNA BR fluorescent dye concentrate 200x, buffer for preparation of dye working solution, and DNA concentration standards (0 and 100 ng/µL). QuDye dsDNA BR Reagent is selective to double-stranded DNA, so measurement results are not affected by RNA, free nucleotides, or protein contaminants. Other minor contaminants such as salts, detergents, and solvents have a non-significant effect on measurements results. This kit allows accurate dsDNA quantification in the range from 100 pg/µL to 1000 ng/µL for initial sample (DNA amount range for measuring: 2–1000 ng of DNA in 200 µL of the test sample).

The main kit component is QuDye dsDNA BR fluorescent dye, which selectively interacts with DNA and exhibits enhanced fluorescence upon binding to DNA molecule. This kit allows convenient dsDNA quantification in the broad concentration range and does not require a large volume of the initial sample (1–20 µL is required for the assay). All measurements are performed at room temperature and take on average 15–20 minutes (depending on the sample amount). Sample fluorescence is stable for 3 hours. To perform a measurement, prepare dye working solution, dilute DNA standards and experimental samples with it in individual tubes and perform fluorescence measurements using fluorometer. For your convenience, we offer kits including thin-wall tubes compatible with fluorometer.

This kit can be also used with QuDye Protein Quantification Kit to determine protein contaminants in the DNA sample.

For quantification of dsDNA of lower concentrations, use QuDye dsDNA HS Assay Kit with the range of initial sample concentrations of 10 pg/µL to 100 ng/µL.

Ready-to-use kit manual

Calculator

Customers also purchased with this product

QuDye dsDNA HS Assay Kit

Ready kit optimised for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL

LumiSpin® PLASMID, Plasmid DNA Isolation Spin Kit

Ready-to-use kit for rapid and efficient purification of plasmid DNA from bacterial cultures using spin columns.

QuDye Protein Quantification Kit

Ready-to-use kit for simple, tolerant-to-contaminants protein quantification. The kit includes concentrated assay reagent, dilution buffer, and prediluted BSA standards.

QuDye dsDNA BR Reagent, 200×

Concentrated reagent for quantitation of double-stranded DNA in a broad range initial sample concentration using a fluorescence plate reader or fluorometer.

General properties

Storage conditions: Store at +4 °C. Warm up to +20 °C before use.
MSDS: Download

Kit

Storage time in months: 12

References

  1. Taira, Y.; Shimoji, Y.; Nakasone, T.; Arakaki, Y.; Nakamoto, T.; Kinjo, T.; Kudaka, W.; Mekaru, K.; Aoki, Y. A High-Risk Gestational Trophoblastic Neoplasia Derived from a Complete Hydatidiform Mole with Coexisting Fetus Identified by Short Tandem Repeats Analysis: A Case Report. Case Reports in Women’s Health, 2021, 31, e00336. doi: 10.1016/j.crwh.2021.e00336
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.