QuDye Protein Quantification Kit

Cat. #	Quantity	Price	Lead time
A5102	30 assays	–	1 days
15102	100 assays	$110.00	1 days
16102	100 assays (incl. tubes)	$130.00	1 days
25102	500 assays	$350.00	1 days

Sample request

The kit includes concentrated 200x assay reagent, dilution buffer, and protein standards 0, 200 and 400 ng/uL optimised for the area of measurements. The assay has a low variability depending on the protein type and is tolerant to contaminants, as reducing agents, nucleotides, free amino acids DNA, but not detergents. This kit allows accurate protein quantification in the range from 12.5 µg/mL to 5 mg/mL for initial sample (protein amount range for measuring using fluorometer: 0.25-5 µg of protein in 200 µL of the test sample). The experiment is performed at room temperature, and the signal is stable for 3 hours.

The quality of measurements can be even higher when the same buffer is used to calculate the background and to prepare the sample.

This kit allows to simply dilute the reagent concentrate and start your measurements. Fluorometer instrument allows to read samples with volume from 1–20 uL.

Ready-to-use kit manual

QuDye Protein Quantification Kit manual

Calculator

Solution calculator

General properties

Storage conditions:	Store at +4 °С. Warm to RT before use.
MSDS:	Download

Kit

Storage time in months:

References

Semenova, D.; Zabirnyk, A.; Lobov, A.; Boyarskaya, N.; Kachanova, O.; Uspensky, V.; Zainullina, B.; Denisov, E.; Gerashchenko, T.; Kvitting, J.-P. E.; Kaljusto, M.-L.; Thiede, B.; Kostareva, A.; Stensløkken, K.-O.; Vaage, J.; Malashicheva, A. Multi-Omics of in Vitro Aortic Valve Calcification. Frontiers in Cardiovascular Medicine, 2022, 9, 1043165. doi: 10.3389/fcvm.2022.1043165
Kotova, A. V.; Lobov, A. A.; Dombrovskaya, J. A.; Sannikova, V. Y.; Ryumina, N. A.; Klausen, P.; Shavarda, A. L.; Malashicheva, A. B.; Enukashvily, N. I. Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome. Biomedicines, 2021, 9(11), 1606. doi: 10.3390/biomedicines9111606