QuDye® Protein Quantification Kit

Fluorometry Protein quantification

Cat. #	Quantity	Price	Lead time
A5102	30 assays	–	1 days
15102	100 assays	$110	1 days
16102 / includes tubes	100 assays (incl. tubes)	$130	1 days
25102	500 assays	$350	1 days

Sample request

Found better price? Let us know and we will propose the way forward!

The kit includes concentrated 200x assay reagent, dilution buffer, and protein standards 0, 200, and 400 ng/uL optimized for the area of measurements. The assay has a low variability depending on the protein type and is tolerant to contaminants, such as reducing agents, nucleotides, and free amino acids DNA, but not detergents. This kit allows accurate protein quantification in the range from 12.5 µg/mL to 5 mg/mL for the initial sample (protein amount range for measuring using fluorometer: 0.25-5 µg of protein in 200 µL of the test sample). The experiment is performed at room temperature, and the signal is stable for 3 hours.

The quality of measurements can be even higher when the same buffer is used to calculate the background and to prepare the sample.

This kit allows simple diluting of the reagent concentrate and starting the measurements. Fluorometer instrument allows to read samples with volume from 1–20 uL.

Ready-to-use kit manual

QuDye® Protein Quantification Kit manual

Calculator

Solution calculator

Customers also purchased with this product

ProteOrange® Protein Quantification Reagent, 500×

ProteOrange® is a fluorescent protein-sensitive dye used for quantitating purified protein samples. The dye shows minimal variability for different proteins. This reagent is a 500× concentrate of ProteOrange dye.

General properties

Storage conditions:	Store at 4 °С. Warm up to room temperature before use. Transportation: at room temperature for up to 3 weeks.
MSDS:	Download

Kit

Storage time in months:

References

Shishkova, D.; Lobov, A.; Repkin, E.; Markova, V.; Markova, Y.; Sinitskaya, A.; Sinitsky, M.; Kondratiev, E.; Torgunakova, E.; Kutikhin, A. Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells. Journal of Cardiovascular Development and Disease, 2024, 11(1), 5. doi: 10.3390/jcdd11010005
Sinitsky, M.; Repkin, E.; Sinitskaya, A.; Markova, V.; Shishkova, D.; Barbarash, O. Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C: Key Proteins and Pathways Underlying Genotoxic Stress-Induced Endothelial Dysfunction. International Journal of Molecular Sciences, 2024, 25(7), 4044. doi: 10.3390/ijms25074044
Grumov, D.; Kostarnoy, A.; Gancheva, P.; Kondratev, A. A Simple and Rapid Microscale Method for Isolating Bacterial Lipopolysaccharides. International Journal of Molecular Sciences, 2024, 25(12), 6345. doi: 10.3390/ijms25126345
Frolov, A.; Lobov, A.; Kabilov, M.; Zainullina, B.; Tupikin, A.; Shishkova, D.; Markova, V.; Sinitskaya, A.; Grigoriev, E.; Markova, Y.; Kutikhin, A. Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity. International Journal of Molecular Sciences, 2023, 24(19), 15032. doi: 10.3390/ijms241915032

Show more (7)

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.