QuDye Protein Quantification Kit

Cat. #	Quantity	Price	Lead time
A5102	30 assays	–	1 days
15102	100 assays	$110	1 days
16102	100 assays (incl. tubes)	$130	1 days
25102	500 assays	$350	1 days

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The kit includes concentrated 200x assay reagent, dilution buffer, and protein standards 0, 200, and 400 ng/uL optimized for the area of measurements. The assay has a low variability depending on the protein type and is tolerant to contaminants, such as reducing agents, nucleotides, and free amino acids DNA, but not detergents. This kit allows accurate protein quantification in the range from 12.5 µg/mL to 5 mg/mL for the initial sample (protein amount range for measuring using fluorometer: 0.25-5 µg of protein in 200 µL of the test sample). The experiment is performed at room temperature, and the signal is stable for 3 hours.

The quality of measurements can be even higher when the same buffer is used to calculate the background and to prepare the sample.

This kit allows simple diluting of the reagent concentrate and starting the measurements. Fluorometer instrument allows to read samples with volume from 1–20 uL.

Ready-to-use kit manual

QuDye Protein Quantification Kit manual

Calculator

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General properties

Storage conditions:	Store at +4 °С. Warm to RT before use.
MSDS:	Download

Kit

Storage time in months:

References

Shishkova, D.; Lobov, A.; Repkin, E.; Markova, V.; Markova, Y.; Sinitskaya, A.; Sinitsky, M.; Kondratiev, E.; Torgunakova, E.; Kutikhin, A. Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells. Journal of Cardiovascular Development and Disease, 2024, 11(1), 5. doi: 10.3390/jcdd11010005
Sinitsky, M.; Repkin, E.; Sinitskaya, A.; Markova, V.; Shishkova, D.; Barbarash, O. Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C: Key Proteins and Pathways Underlying Genotoxic Stress-Induced Endothelial Dysfunction. International Journal of Molecular Sciences, 2024, 25(7), 4044. doi: 10.3390/ijms25074044
Frolov, A.; Lobov, A.; Kabilov, M.; Zainullina, B.; Tupikin, A.; Shishkova, D.; Markova, V.; Sinitskaya, A.; Grigoriev, E.; Markova, Y.; Kutikhin, A. Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity. International Journal of Molecular Sciences, 2023, 24(19), 15032. doi: 10.3390/ijms241915032
Semenova, D.; Zabirnyk, A.; Lobov, A.; Boyarskaya, N.; Kachanova, O.; Uspensky, V.; Zainullina, B.; Denisov, E.; Gerashchenko, T.; Kvitting, J.-P. E.; Kaljusto, M.-L.; Thiede, B.; Kostareva, A.; Stensløkken, K.-O.; Vaage, J.; Malashicheva, A. Multi-Omics of in Vitro Aortic Valve Calcification. Frontiers in Cardiovascular Medicine, 2022, 9, 1043165. doi: 10.3389/fcvm.2022.1043165