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QuDye dsDNA HS Assay kit (optimized for Qubit®)

QuDye dsDNA HS Assay kit (optimized for Qubit®)

Cat. #	Quantity	Price	Lead time	Buy this product
12102	100 assays	$55.00	in stock
13102	100 assays (incl. tubes)	$70.00	in stock

This kit for DNA quantification is optimized for Qubit® fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL.The kit includes the concentrated QuDye HS assay reagent, dilution buffer, and a 10 ng/uL DNA standard optimized to cover the middle of the measurements range. The assay is selective towards dsDNA (double-stranded DNA) over RNA and is accurate for initial sample concentrations from 10 pg/uL to 100 ng/uL. The experiment is performed at room temperature and the signal is stable for 3 hours. The assay tolerates contaminants like salts, free nucleotides, detergents, or proteins.

A second standard for Qubit® instruments is not required due to the fact that the original Qubit® protocol recommends to use 0 ng/uL in TE buffer as a standard which can easily be prepared from the TE buffer concentrate provided. The quality of the measurement can be even higher when the same buffer is used for sample preparation and background measurement.

This kit allows to simply dilute the reagent and buffer concentrates and start your measurements. The Qubit® instrument is able to read samples of about 200 uL. For sample preparation mix 180-199 uL of the QuDye HS working solution and 20-1 uL of the DNA sample to be quantified.