QuDye® dsDNA HS Assay Kit

Fluorometry Nucleic acid quantification

Cat. #	Quantity	Price	Lead time
A2102	10 assays	–	1 days
12102	100 assays	$105 $67	21 days
13102 / includes tubes	100 assays	$125 $79	21 days
52102	500 assays	$290 $147	21 days
53102 / includes tubes	500 assays	$389 $176	21 days
72102	1000 assays	$499	21 days

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This kit for DNA quantification is optimized for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL.The kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/uL). The assay is selective towards dsDNA (double-stranded DNA) over RNA and is accurate for initial sample concentrations from 10 pg/uL to 100 ng/uL. The experiment is performed at room temperature and the signal is stable for 3 hours. The assay tolerates contaminants like salts, free nucleotides, detergents, or proteins.

This kit allows to simply dilute the reagent and buffer concentrates and start your measurements. The fluorometer instrument is able to read samples of about 200 uL. For sample preparation mix 180-199 uL of the QuDye HS working solution and 20-1 uL of the DNA sample to be quantified.

Ready-to-use kit manual

QuDye® dsDNA HS Assay Kit manual

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General properties

Storage conditions:	Store at 4 °С. Warm up to room temperature before use. Transportation: at room temperature for up to 3 weeks.
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References

Bonanno Ferraro, G.; Bonomo, C.; Brandtner, D.; Mancini, P.; Veneri, C.; Briancesco, R.; Coccia, A.M.; Lucentini, L.; Suffredini, E.; Bongiorno, D.; Musso, N.; Stefani, S.; La Rosa, G. Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR. Science of The Total Environment, 2024, 933, 173217. doi: 10.1016/j.scitotenv.2024.173217
Grumov, D.; Kostarnoy, A.; Gancheva, P.; Kondratev, A. A Simple and Rapid Microscale Method for Isolating Bacterial Lipopolysaccharides. International Journal of Molecular Sciences, 2024, 25(12), 6345. doi: 10.3390/ijms25126345
Frolov, A.V.; Vishnevskaya, M.S.; Viljanen, H.; Montreuil, O.; Akhmetova, L.A. New data on trophic associations of dung beetles ( Coleoptera , Scarabaeidae , Scarabaeinae ) and lemurs ( Primates , Lemuroidea ) in Madagascar revealed by metabarcoding. Biodiversity Data Journal, 2024, 12, e130400. doi: 10.3897/BDJ.12.e130400
Wu, Y.; Leyk, S.; Torabi, H.; Höhn, K.; Honecker, B.; Tauler, M. D. P. M.; Cadar, D.; Jacobs, T.; Bruchhaus, I.; Metwally, N. G. Plasmodium Falciparum Infection Reshapes the Human MicroRNA Profiles of Red Blood Cells and Their Extracellular Vesicles. iScience, 2023, 26(7), 107119. doi: 10.1016/j.isci.2023.107119

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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.