Ribo488 RNA BR Fluorimetric Assay Kit

Fluorometry Nucleic acid quantification

Cat. #	Quantity	Price	Lead time
S2502	40 assays	–	1 days
12502	100 assays	$239.00	1 days
52502	500 assays	$899.00	21 days

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The Ribo488 RNA BR Fluorimetric Assay Kit is designed for measuring RNA concentration over a wide range using a fluorimeter (any fluorimeter is suitable; for example, QuReader 1 or QuReader 8).

The kit allows accurate measurement of RNA concentration in the range of 1–1000 ng/µL (RNA quantity for measurement on the fluorimeter: 20–1200 ng of RNA per 200 µL of sample).

The main component of the kit is the fluorescent dye Ribo488, which binds to RNA and exhibits pronounced fluorescence upon complexation. Impurities in small amounts, such as salts, detergents, and solvents, have only a negligible effect on measurement results.

Using the Ribo488 RNA BR Fluorimetric Assay kit is a convenient way to measure RNA over a wide range of concentrations:

It requires a small volume of the original sample (1–20 µL);
All measurements are performed at room temperature;
Depending on the number of samples, measurements take an average of 15–20 minutes;
The fluorescence of the samples is stable for 3 hours.

The kit includes a 200× concentrate of the fluorescent dye Ribo488, a buffer for preparing the dye's working solution, and RNA Concentration Standards (0 and 100 ng/µL). For convenience, our kit contains thin-walled tubes compatible with a fluorimeter.

To determine protein impurities in the RNA sample, the QuDye® Protein quantification kit can be used.

Ready-to-use kit manual

Ribo488 RNA BR Fluorimetric Assay Kit manual

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General properties

Storage conditions:	Store at 4 °C. Warm up to RT before use. Transportation: at room temperature for up to 1 week.
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Kit

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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.