LumiSpin UNI, DNA Isolation Spin Kit for Any Sample

DNA isolation
Cat. # Quantity Price Lead time
11573
10 minipreps
–   1 days
21573
50 minipreps
$195 1 days
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The kit is designed to isolate genomic DNA from a wide range of biological samples using spin columns. The kit includes all the necessary components. First, cell lysis is performed using Lysis Solution and Proteinase K. The lysate is mixed with Sorption Solution that allows selective binding of DNA to the membrane in the column. In the following step, a series of washings of bound DNA is performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the genomic DNA is eluted with Elution Buffer or, if necessary, with deionized water. The kit also includes corundum and pestles for homogenization of plant and animal tissues, RNase A for efficient removal of any residual RNA, Red Blood Cell Lysis Buffer (in case of prior separation of leukocytes, which provides increased DNA yield compared to DNA purification from a whole blood sample).

Kit Specifications:

  • Samples: plant and animal tissues and organs, whole blood, buccal swabs, leukocytes, cultured mammalian cells, and gram-negative bacteria
  • Operation time: 30 minutes
  • Column binding capacity: 20 µg DNA
  • DNA typical yield: plant and animal tissues – 3–20 µg (depending on the sample), whole blood (100 µL) – 1–3 µg, leukocytes (isolated from 1 mL of whole blood) – 4–5 µg, buccal swab – 0.5–2 µg, cultured cells (1 × 106) – 3–6 µg, gram-negative bacteria (1 × 108) – 5–10 µg
  • DNA quality: 30–50 kb in size, OD 260/280 1.7–1.8

The isolated DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.

LumiSpin UNI, DNA Isolation Spin Kit for Any Sample, 21573

Product in action

Electrophoregram of genomic DNA isolated from various sources using LumiSpin UNI Kit

Amplification curves of the human β-actin gene fragment

Ready-to-use kit manual

Calculator

General properties

Storage conditions: Store at temperature 20°С.
MSDS: Download

Kit

Storage time in months: 12

References

  1. Fadeev, A.Y..; Gavirova, L.A.; Georgieva, M.L.; Kozlovsky, V.V.; Simakova, U.V.; Shestakov, A.I.; Bubnova, E.N. Effect of Hydrocarbon Pollution on the Fungal Communities of the Littoral Sediments of the White and Barents Seas. Microbiology, 2024, 93, 51-60. doi: 10.1134/s002626172360324x
  2. Chetverikov, P. E.; Craemer, C.; Gankevich, V. D.; Zhuk, A. S. Integrative Taxonomy of the Gall Mite Nothopoda Todeica n. Sp. (Eriophyidae) from the Disjunct Afro-Australasian Fern Todea Barbara: Morphology, Phylogeny, and Mitogenomics. Insects, 2023, 14(6), 507. doi: 10.3390/insects14060507
  3. Ianshina, T.; Sidorin, A.; Petrova, K.; Shubert, M.; Makeeva, A.; Sambuk, E.; Govdi, A.; Rumyantsev, A.; Padkina, M. Effect of Methionine on Gene Expression in Komagataella Phaffii Cells. Microorganisms, 2023, 11(4), 877. doi: 10.3390/microorganisms11040877
  4. Makeeva, A.; Muzaev, D.; Shubert, M.; Ianshina, T.; Sidorin, A.; Sambuk, E.; Rumyantsev, A.; Padkina, M. Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains. Microorganisms, 2023, 11(9), 2297. doi: 10.3390/microorganisms11092297
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