Alkyne dT phosphoramidite

This product is discontinued, and no longer available.
 We recommend Alkyne dT phosphoramidite as a substitute
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This product will be discontinued soon in favor of Alkyne dT phosphoramidite. We may still have some material in stock (please see availability above).

Alkyne dT phosphoramidite is used for the synthesis of oligonucleotides containing an internal alkyne modification attached to the uridine 5-position. The alkyne group can be subsequently modified by various azides using copper-catalyzed Click chemistry.

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DusQ 1 phosphoramidite

A black quencher dye phosphoramidite for the synthesis of oligonucleotide probes labeled with DusQ 1 quenchers at 5’-terminal. Useful for HEX, JOE, ROX, Cyanine3.
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DusQ 2 phosphoramidite

DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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General properties

Appearance: white solid
Molecular weight: 935.01
Molecular formula: C50H59N6O10P
Solubility: good in THF, DCM, acetonitrile
Quality control: NMR 1H, HPLC-MS (90+%), functional testing in oligo synthesis
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Coupling conditions: 6 min. coupling time; 45 sec. oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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