Found better price?Let us knowand we will propose the way forward!
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA
(carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange
spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor
(and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their
spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which
contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end
(using Fluorescein dT Phosphoramidite and FAM
CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR
and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection
channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and
sterically non-hindered primary amines, so it is strongly recommended to follow specified
conditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
Alkyne phosphoramidite for the synthesis of oligonucleotides ready for click chemistry.…
General properties
Appearance:
purple solid
Mass spec M+ increment:
589.60
Molecular weight:
727.83
Molecular formula:
C40H50N5O6P
Solubility:
good in acetonitrile, dichloromethane
Quality control:
NMR 1H, HPLC-MS (95%), coupling test
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.