TAMRA CPG 500, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2792-100mg | 100 mg | $95 | in stock | |
| 2792-500mg | 500 mg | $295 | in stock | |
| 2792-1g | 1 g |
$469
|
in stock | |
| 2792-5g | 5 g | please inquire | in stock |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
Customers also purchased with this product
Cyanine5.5 azide
Far red / near infrared fluorescent Cyanine5.5 dye derivative for click chemistry.ROX azide, 6-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 6-isomer.FAM CPG 500, 6-isomer
Controlled pore glass for the synthesis of modified oligonucleotides containing 3'-fluorescein (FAM) modification.
Add this product to your cart andget free express delivery
General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−70 |
Product citations
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
Your cart is empty
$ 
