DusQ® 21 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3151-100mg | 100 mg | – | in stock | |
| 3151-1g | 1 g |
$990
|
in stock |
DusQ® 21 is a quencher for the dyes in far red region of the spectrum. The dye is a xanthene derivative, unlike DusQ 1 or DusQ 2, that are azo dyes. It has a high extinction coefficient, and provides an outstanding quenching efficiency.
The controlled pore glass solid support allows to synthesize oligonucleotides carrying the dye moiety on 3’-terminus.
We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of DusQ 21 quencher can be used.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or potassium carbonate K2CO3 (50 mM solution in methanol).
Absorption spectrum of DusQ 21
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Controlled pore glass (CPG) 500 solid support for synthesizing oligonucleotides with 3'-terminal DusQ® 1 quencher. Compatible with standard deblocking conditions.
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Azide of green-yellow emitting dye SIMA for conjugation with oligonucleotides via [3+2]-dipolar cycloaddition.SIMA phosphoramidite, 6-isomer
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General properties
| Appearance: | dark blue beads |
| Quality control: | 1H NMR of bound reagent, coupling testing with HPLC-MS |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 656 |
| ε, L⋅mol−1⋅cm−1: | 72800 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/91
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