FAM amine, 5-isomer

Cat. # Quantity Price Lead time
141C0 1 mg $110.00 in stock
241C0 5 mg $210.00 in stock
441C0 25 mg $410.00 in stock
541C0 50 mg $695.00 in stock
641C0 100 mg $1190.00 in stock

Fluorescein (FAM) amine, isomerically pure dye (5-isomer). Unlike fluoresceinamine, this reagent contains a linker arm with an aliphatic amine group that is more reactive. It can react with electrophilic reagents like activated esters or epoxides, and be involved in enzymatic transamination.

FAM absorbance and emission spectra

FAM absorbance and emission spectra

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Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

General properties

Appearance: yellow solid
Molecular weight: 510.97
CAS number: 2183440-41-5 (hydrochloride), 138589-19-2
Molecular formula: C27H27N2ClO6
IUPAC name: 6-aminohexylaminocarbonylfluorescein hydrochloride
Solubility: good in methanol, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17
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