DusQ 1 amidite, 5'-terminal

Cat. # Quantity Price Lead time
1D260 100 mg $290 in stock
2D260 250 mg $590 in stock
3D260 500 mg $990 in stock
4D260 1 g $1690 in stock
6D260 5 g $6790 in stock
7D260 10 g please inquire in stock
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DusQ 1 is a fluorescence quencher with the most effective absorption in the range of 480–580 nm, the maximum absorption is at 522 nm.

As it was demonstrated for a complete analogue of this quencher, both dynamic (FRET) and static fluorescence quenching can be described [1-2]. For this reason, DusQ 1 can be used in hybridization probes such as TaqMan, Molecular Beacon, Scorpion to quench the fluorescence of a wide range of fluorophores including FAM, JOE, VIC, R6G, HEX, TET.

The use of nonfluorescent quenchers as FRET pair acceptors has many advantages compared to the use of fluorophores as quenchers. As part of the probe, the DusQ 1 chromophore more efficiently absorbs the fluorescence of the FRET-pair donor, which makes it possible to significantly reduce the background fluorescence of the probe and, thus, increase the signal-to-noise ratio and increase the dynamic range of the signal.

Probes based on DusQ 1 are conveniently used in multiplex analysis, since this quencher, unlike fluorescent FRET acceptors, does not possess its own fluorescence and does not «occupy» the detecting channels available to the researcher.

Usage

Coupling: 4 minutes

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1).

Absorption spectrum of DusQ 1

Absorption spectrum of DusQ 1

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General properties

Appearance: dark colored solid
Molecular weight: 676.75
Molecular formula: C34H45N8O5P
Solubility: good in acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), coupling test
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 27300
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Diluent: dry acetonitrile (dissolve to a concentration of 0.1 M, storage of the ready-made solution - 3 days).
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