Universal CPG type I, 500A

Cat. # Quantity Price Lead time
2646-1g 1 g $39.00 in stock
2646-10g 10 g $351.00 21 days

The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.

The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 8 hours at 80 °C using ammonium hydroxide; 17 hours at 55 °C or 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1); 8 hours at 55 °C using 40% methylamine; 30 minutes at 80 °C or 3 hours at 55 °C or 17 hours at room temperature using 0.4M NaOH in methanol - water (4:1).

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General properties

Appearance: white to cream beads
Molecular weight: 564.61
Molecular formula: C31H34*NO9
Quality control: NMR 1H and HPLC-MS (95%), loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−95
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C
Deprotection conditions: identical to protected nucleobases
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