dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11010 | 100 uL | $39.00 | in stock | |
| 41010 | 1 mL | $310.00 | in stock | |
| 51010 | 1.5 mL |
$450.00
|
in stock | |
| 61010 | 2 mL |
$498.00
|
in stock | |
| 71010 | 5 mL |
$845.00
|
in stock | |
| 81010 | 10 mL |
$1190.00
|
in stock | |
| 91010 | 50 mL |
$4450.00
|
in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Excitation and emission spectra of dsDNA complex with dsGreen
Recommended protocol
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| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Carter, J.G.; Iturbe, L.O.; Duprey, J.-L.H.A.; Carter, I.R.; Southern, C.D.; Rana, M.; Bosworth, A. Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR. medRxiv, preprint. doi: 10.1101/2020.12.31.20248236
- Fang, Y.; Coulter, J.A.; Wu, J.; Liu, L.; Li, X.; Dong, Y.; Ma, L.; Pu, Y.; Sun, B.; Niu, Z.; Jin, J.; Zhao, Y.; Mi, W.; Xu, Y.; Sun, W. Identification of differentially expressed genes involved in amino acid and lipid accumulation of winter turnip rape (Brassica rapa L.) in response to cold stress. PloS ONE, 2021, 16(2), e0245494. doi: 10.1371/journal.pone.0245494
- Morozov, V.N.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Comparison of quenching efficacy of SYBR green I and picoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systems. Journal of Molecular Liquids, 2021, 321, 114751. doi: 10.1016/j.molliq.2020.114751
- Bitarishvili, S. V. and Bondarenko, V. S. and Geras'kin, S. A. Expression of Gibberelline Biosynthesis and Catabolism Genes in the Embryos of γ-Irradiated Barley Seeds. Biology Bulletin, 2020, 47(11), 1558–1563. doi: 10.1134/S1062359020110059
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