dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11010 | 100 uL | $39.00 | in stock | |
| 41010 | 1 mL | $310.00 | in stock | |
| 51010 | 1.5 mL |
$450.00
|
in stock | |
| 61010 | 2 mL |
$498.00
|
in stock | |
| 71010 | 5 mL |
$845.00
|
in stock | |
| 81010 | 10 mL |
$1190.00
|
in stock | |
| 91010 | 50 mL |
$4450.00
|
in stock |
dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
qPCR curves with dsGreen
Excitation and emission spectra of dsDNA complex with dsGreen
Recommended protocol
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Water soluble cyanine dye with bright fluorescence, and high extinction coefficient. Contains an amino group for the conjugation with electrophiles, and enzymatic transamination labeling.General properties
| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Carter, J.G.; Iturbe, L.O.; Duprey, J.-L.H.A.; Carter, I.R.; Southern, C.D.; Rana, M.; Bosworth, A. Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR. medRxiv, preprint. doi: 10.1101/2020.12.31.20248236
- Morozov, V.N.; Klimovich, M.A.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Interaction of Gold Nanoparticles with Cyanine Dyes in Cholesteric DNA Submicroparticles. High Energy Chemistry, 2021, 55(5), 341–348. doi: 10.1134/S0018143921050088
- Carter, J.G.; Iturbe, L.O.; Duprey, J.-L.H.A.; Carter, I.R.; Southern, C.D.; Rana, M.; Whalley, C.M.; Bosworth, A.; Beggs, A.D.; Hicks, M.R.; Tucker, J.H.R.; Dafforn, T.R. Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription{\textendash}free exponential amplification reaction, RTF-EXPAR. Proceedings of the National Academy of Sciences of the U.S.A., 2021, 118(35), e2100347118. doi: 10.1073/pnas.2100347118
- Fang, Y.; Coulter, J.A.; Wu, J.; Liu, L.; Li, X.; Dong, Y.; Ma, L.; Pu, Y.; Sun, B.; Niu, Z.; Jin, J.; Zhao, Y.; Mi, W.; Xu, Y.; Sun, W. Identification of differentially expressed genes involved in amino acid and lipid accumulation of winter turnip rape (Brassica rapa L.) in response to cold stress. PloS ONE, 2021, 16(2), e0245494. doi: 10.1371/journal.pone.0245494
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