dsGreen for Real-Time PCR, 100×

Cat. # Quantity Price Lead time
11010 100 uL $39.00 in stock
41010 1 mL $310.00 in stock
51010 1.5 mL $450.00 in stock
61010 2 mL $498.00 in stock
71010 5 mL $845.00 in stock
81010 10 mL $1190.00 in stock
91010 50 mL $4450.00 in stock

dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain

Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

qPCR curves with dsGreen

qPCR curves with dsGreen

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General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 454
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Garafutdinov, R.R.; Sakhabutdinova, A.R.; Kupryushkin, M.S.; Pyshnyi, D.V. Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase. Data in Brief, 2020, 29, 105188. doi: 10.1016/j.dib.2020.105188
  2. Garafutdinov, R.R.; Gilvanov, A.R.; Sakhabutdinova, A.R. The Influence of Reaction Conditions on DNA Multimerization During Isothermal Amplification with Bst exo– DNA Polymerase. Applied Biochemistry and Biotechnology, 2020, 190(2), 758–771. doi: 10.1007/s12010-019-03127-6
  3. Garafutdinov, R.R.; Sakhabutdinova, A.R.; Kupryushkin, M.S.; Pyshnyi, D.V. Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase. Biochimie, 2020, 168, 259–267. doi: 10.1016/j.biochi.2019.11.013
  4. Schwenk, J.; Boudkkazi, S.; Kocylowski, M.K.; Brechet, A.; Zolles, G.; Bus, T.; Costa, K.; Kollewe, A.; Jordan, J.; Bank, J.; Bildl, W.; Sprengel, R.; Kulik, A.; Roeper, J.; Schulte, U.; Fakler, B. An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. Neuron, 2019, 104(4), 680–692.e9. doi: 10.1016/j.neuron.2019.08.033
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