dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11010 | 100 uL | $39.00 | in stock | |
| 41010 | 1 mL | $310.00 | in stock | |
| 51010 | 1.5 mL |
$450.00
|
in stock | |
| 61010 | 2 mL |
$498.00
|
in stock | |
| 71010 | 5 mL |
$845.00
|
in stock | |
| 81010 | 10 mL |
$1190.00
|
in stock | |
| 91010 | 50 mL |
$4450.00
|
in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Recommended protocol
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General properties
| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Morozov, V.N.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Comparison of quenching efficacy of SYBR green I and picoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systems. Journal of Molecular Liquids, in press. doi: 10.1016/j.molliq.2020.114751
- Sapozhnikova, Y.P.; Koroleva, A.G.; Yakhnenko, V.M.; Tyagun, M.L.; Glyzina, O.Y.; Coffin, A.B.; Makarov, M.M.; Shagun, A.N.; Kulikov, V.A.; Gasarov, P.V.; Kirilchik, S.V.; Klimenkov, I.V.; Sudakov, N.P.; Anoshko, P.N.; Kurashova, N.A.; Sukhanova, L.V. Molecular and cellular responses to long-term sound exposure in peled (Coregonus peled). Journal of the Acoustical Society of America, 2020, 148(2), 895–907. doi: 10.1121/10.0001674
- Thompson, T.A.; Touré, M.B.; Sanogo, D.; Shaffer, J.G.; Doumbia, S.O.; Krogstad, D.J. Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals. Malaria Journal, 2020, 19, 295. doi: 10.1186/s12936-020-03365-8
- Garafutdinov, R.R.; Gilvanov, A.R.; Kupova, O.Y.; Sakhabutdinova, A.R. Effect of metal ions on isothermal amplification with Bst exo- DNA polymerase. International Journal of Biological Macromolecules, 2020, 161, 1447–1455. doi: 10.1016/j.ijbiomac.2020.08.028
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