dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41010 | 1 mL | $310.00 | in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Recommended protocol
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| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Revalde, J.L.; Li, Y.; Wijeratne, T.S.; Bugde, P.; Hawkins, B.C.; Rosengren, R.J.; Paxton, J.W. Curcumin and its cyclohexanone analogue inhibited human Equilibrative nucleoside transporter 1 (ENT1) in pancreatic cancer cells. European Journal of Pharmacology, 2017, 803, 167–173. doi: 10.1016/j.ejphar.2017.03.055
- Wu, Z.-G.; Jiang, W.; Chen, S.-L.; Mantri, N.; Tao, Z.-M.; Jiang, C.-X. Insights from the Cold Transcriptome and Metabolome of Dendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation. Frontiers in Plant Science, 2016, 7, 1653. doi: 10.3389/fpls.2016.01653
- Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J. Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community. Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795
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