dsGreen for Real-Time PCR, 100×

Cat. # Quantity Price Lead time
A1010 50 uL –   in stock
11010 100 uL $39 in stock
41010 1 mL $235 in stock
71010 5 mL $510 in stock
91010 50 mL $2670 in stock
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dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain

Excitation and emission spectra of dsDNA complex with dsGreen

Excitation and emission spectra of dsDNA complex with dsGreen

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General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Bykonia, E.N.; Kleymenov, D.A.; Gushchin, V.A.; Sinyavin, A.E.; Mazunina, E.P.; Kuznetsova, N.A.; Kozlova, S.R.; Zolotar, A.N.; Shidlovskaya, E.V.; Usachev, E.V.; Pochtovyi, A.A.; Kustova, D.D.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Major role of S-glycoprotein in providing immunogenicity and protective immunity in mRNA lipid nanoparticle vaccines based on SARS-CoV-2 structural proteins. bioRxiv, 2024, preprint. doi: 10.1101/2023.12.30.573713
  2. Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
  3. Sakhabutdinova, A. R.; Chemeris, A. V.; Garafutdinov, R. R. Detection of Specific RNA Targets by Multimerization. Biochemistry (Moscow), 2023, 88(5), 679–686. doi: 10.1134/S0006297923050103
  4. Hildebrandt, R.P.; Moss, K.R.; Janusz-Kaminska, A.; Knudson, L.A.; Denes, L.T.; Saxena, T.; Boggupalli, D.P.; Li, Z.; Lin, K.; Bassell, G.J.; Wang, E.T. Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes. Nature Communications, 2023, 14, 3427. doi: 10.1038/s41467-023-38923-6
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