dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11010 | 100 uL | $39.00 | in stock | |
| 41010 | 1 mL | $310.00 | in stock | |
| 51010 | 1.5 mL |
$450.00
|
in stock | |
| 61010 | 2 mL |
$498.00
|
in stock | |
| 71010 | 5 mL |
$845.00
|
in stock | |
| 81010 | 10 mL |
$1190.00
|
5 days | |
| 91010 | 50 mL |
$4450.00
|
in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Recommended protocol
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| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Schwenk, J.; Boudkkazi, S.; Kocylowski, M.K.; Brechet, A.; Zolles, G.; Bus, T.; Costa, K.; Kollewe, A.; Jordan, J.; Bank, J.; Bildl, W.; Sprengel, R.; Kulik, A.; Roeper, J.; Schulte, U.; Fakler, B. An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. Neuron, in press. doi: 10.1016/j.neuron.2019.08.033
- Garafutdinov, R.R.; Gilvanov, A.R.; Sakhabutdinova, A.R. The Influence of Reaction Conditions on DNA Multimerization During Isothermal Amplification with Bst exo– DNA Polymerase. Applied Biochemistry and Biotechnology, in press. doi: 10.1007/s12010-019-03127-6
- Yang, F.; Teoh, F.; Tan, A.S.M.; Cao, Y.; Pavelka, N.; Berman, J. Aneuploidy enables cross-adaptation to unrelated drugs. Molecular Biology and Evolution, 2019, 36(8), 1768–1782. doi: 10.1093/molbev/msz104
- Segal, E.S.; Gritsenko, V.; Levitan, A.; Yadav, B.; Dror, N.; Steenwyk, J.L.; Silberberg, Y.; Mielich, K.; Rokas, A.; Gow, N.A.R.; Kunze, R.; Sharan, R.; Berman, J. Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans. mBio, 2018, 9(5), e02048-18. doi: 10.1128/mBio.02048-18
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