dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11010 | 100 uL | $39.00 | in stock | |
| 41010 | 1 mL | $310.00 | in stock | |
| 51010 | 1.5 mL |
$450.00
|
in stock | |
| 61010 | 2 mL |
$498.00
|
in stock | |
| 71010 | 5 mL |
$845.00
|
in stock | |
| 81010 | 10 mL |
$1190.00
|
in stock | |
| 91010 | 50 mL |
$4450.00
|
in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Recommended protocol
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Water-soluble NIR dye amine, sulfonated derivative of Cyanine7 dye, for the modification via enzymatical transamination.General properties
| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Garafutdinov, R.R.; Sakhabutdinova, A.R.; Kupryushkin, M.S.; Pyshnyi, D.V. Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase. Data in Brief, 2020, 29, 105188. doi: 10.1016/j.dib.2020.105188
- Garafutdinov, R.R.; Gilvanov, A.R.; Sakhabutdinova, A.R. The Influence of Reaction Conditions on DNA Multimerization During Isothermal Amplification with Bst exo– DNA Polymerase. Applied Biochemistry and Biotechnology, 2020, 190(2), 758–771. doi: 10.1007/s12010-019-03127-6
- Garafutdinov, R.R.; Sakhabutdinova, A.R.; Kupryushkin, M.S.; Pyshnyi, D.V. Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase. Biochimie, 2020, 168, 259–267. doi: 10.1016/j.biochi.2019.11.013
- Schwenk, J.; Boudkkazi, S.; Kocylowski, M.K.; Brechet, A.; Zolles, G.; Bus, T.; Costa, K.; Kollewe, A.; Jordan, J.; Bank, J.; Bildl, W.; Sprengel, R.; Kulik, A.; Roeper, J.; Schulte, U.; Fakler, B. An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. Neuron, 2019, 104(4), 680–692.e9. doi: 10.1016/j.neuron.2019.08.033
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