sulfo-Cyanine5 hydrazide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13370 | 1 mg | $125 | in stock | |
| 23370 | 5 mg | $325 | in stock | |
| 43370 | 25 mg |
$850
|
in stock | |
| 53370 | 50 mg |
$1490
|
in stock | |
| 63370 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5.
Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
sulfo-Cyanine5 absorbance and emission spectra
Customers also purchased with this product
FAM hydrazide, 5-isomer
FAM hydrazide is a carbonyl reactive fluorescein derivative. Pure 5-isomer. Can label oxidized proteins, saccharides, and many small molecules with carbonyl groups.Cyanine5 hydrazide
Cy5 hydrazide is a reactive dye for aldehydes and ketones. It can be used for the labeling of glycoproteins after periodate oxidation of sugar moieties.AF 647 maleimide
AF 647 maleimide is a thiol-reactive far-red fluorescent dye for labeling of thiolated biomolecules (proteins, oligonucleotides, etc.).General properties
| Appearance: | dark blue solid |
| Molecular weight: | 656.83 |
| CAS number: | 2055138-61-7 |
| Molecular formula: | C32H40N4O7S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Your cart is empty
$ 
