FAM hydrazide, 5-isomer

Cat. # Quantity Price Lead time
14170 1 mg $110 in stock
24170 5 mg $210 in stock
44170 25 mg $410 in stock
54170 50 mg $695 in stock
64170 100 mg $1190 in stock
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Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: yellow solid
Mass spec M+ increment: 372.07
Molecular weight: 426.81
CAS number: 2183440-64-2
Molecular formula: C21H15N2ClO6
IUPAC name: Spiro[isobenzofuran-​1(3H)​,​9'-​[9H]​xanthene]​-​5-​carboxylic acid, 3',​6'-​dihydroxy-​3-​oxo-​, hydrazide, hydrochloride
Solubility: good in ethanol, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
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