FAM hydrazide, 5-isomer

Cat. # Quantity Price Lead time
14170 1 mg $110.00 in stock
24170 5 mg $210.00 in stock
44170 25 mg $410.00 in stock
54170 50 mg $695.00 in stock
64170 100 mg $1190.00 in stock

Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: yellow solid
Mass spec M+ increment: 372.07
Molecular weight: 426.81
CAS number: 2183440-64-2
Molecular formula: C21H15N2ClO6
IUPAC name: Spiro[isobenzofuran-​1(3H)​,​9'-​[9H]​xanthene]​-​5-​carboxylic acid, 3',​6'-​dihydroxy-​3-​oxo-​, hydrazide, hydrochloride
Solubility: good in ethanol, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17
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