ROX maleimide, 5-isomer

Cat. # Quantity Price Lead time
11280 1 mg $110.00 in stock
21280 5 mg $210.00 in stock
41280 25 mg $410.00 in stock
51280 50 mg $695.00 in stock
61280 100 mg $1190.00 in stock

ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.

This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.

Absorption and emission spectra of ROX

Absorption and emission spectra of ROX

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General properties

Appearance: red powder
Mass spec M+ increment: 656.3
Molecular weight: 656.73
Molecular formula: C39H36N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00
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