ROX maleimide, 5-isomer

Cat. # Quantity Price Lead time
11280 1 mg $110 in stock
21280 5 mg $165 in stock
41280 25 mg $285 in stock
51280 50 mg $495 in stock
61280 100 mg $880 in stock
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ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.

This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.

Absorption and emission spectra of ROX

Absorption and emission spectra of ROX

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BDP R6G maleimide

BDP R6G is a borondipyrromethene dye tuned to R6G (rhodamine 6G) channel. This is a thiol reactive maleimide for the labeling of protein cysteine residues, and other thiols.

ROX azide, 5-isomer

Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.

Cyanine5 maleimide

Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.

General properties

Appearance: red powder
Mass spec M+ increment: 656.3
Molecular weight: 656.73
Molecular formula: C39H36N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00
CF260: 0.62
CF280: 0.49

Product citations

  1. Khan, S.; Molloy, J.E.; Puhl, H.; Schulman, H.; Vogel, S.S. Real-time single-molecule imaging of CaMKII-calmodulin interactions. Biophysical Journal, 2024, 123(7), 824-838. doi: 10.1016/j.bpj.2024.02.021
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