ROX maleimide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11280 | 1 mg | $110 | in stock | |
| 21280 | 5 mg | $165 | in stock | |
| 41280 | 25 mg | $285 | in stock | |
| 51280 | 50 mg |
$495
|
in stock | |
| 61280 | 100 mg |
$880
|
in stock |
ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.
This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.
Absorption and emission spectra of ROX
Recommended protocol
Calculator
Customers also purchased with this product
Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.AF 488 NHS ester
AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.Cyanine7 maleimide
Cyanine7 maleimide is a sulfhydryl-reactive NIR dye for labeling of proteins.General properties
| Appearance: | red powder |
| Mass spec M+ increment: | 656.3 |
| Molecular weight: | 656.73 |
| Molecular formula: | C39H36N4O6 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 570 |
| ε, L⋅mol−1⋅cm−1: | 93000 |
| Emission maximum, nm: | 591 |
| Fluorescence quantum yield: | 1.00 |
| CF260: | 0.62 |
| CF280: | 0.49 |
Product citations
- Khan, S.; Molloy, J.E.; Puhl, H.; Schulman, H.; Vogel, S.S. Real-time single-molecule imaging of CaMKII-calmodulin interactions. Biophysical Journal, 2024, 123(7), 824-838. doi: 10.1016/j.bpj.2024.02.021
The count of items is incorrect.
Your cart is empty
$ 
