ROX maleimide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11280 | 1 mg | $110 | in stock | |
| 21280 | 5 mg | $165 | in stock | |
| 41280 | 25 mg | $285 | in stock | |
| 51280 | 50 mg |
$495
|
in stock | |
| 61280 | 100 mg |
$880
|
in stock |
ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.
This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.
Absorption and emission spectra of ROX
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| Appearance: | red powder |
| Mass spec M+ increment: | 656.3 |
| Molecular weight: | 656.73 |
| Molecular formula: | C39H36N4O6 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 570 |
| ε, L⋅mol−1⋅cm−1: | 93000 |
| Emission maximum, nm: | 591 |
| Fluorescence quantum yield: | 1.00 |
| CF260: | 0.62 |
| CF280: | 0.49 |
Product citations
- Khan, S.; Molloy, J.E.; Puhl, H.; Schulman, H.; Vogel, S.S. Real-time single-molecule imaging of CaMKII-calmodulin interactions. Biophysical Journal, 2024, 123(7), 824-838. doi: 10.1016/j.bpj.2024.02.021
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