dsGreen for Real-Time PCR, 100×

PCR and qPCR
Cat. # Quantity Price Lead time
A1010 50 uL –   in stock
11010 100 uL $39 in stock
41010 1 mL $235 in stock
71010 5 mL $510 in stock
91010 50 mL $2670 in stock
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dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain
dsGreen for Real-Time PCR, 100×
dsGreen structure
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Excitation and emission spectra of dsDNA complex with dsGreen

Excitation and emission spectra of dsDNA complex with dsGreen

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General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Bykonia, E.N.; Kleymenov, D.A.; Gushchin, V.A.; Sinyavin, A.E.; Mazunina, E.P.; Kuznetsova, N.A.; Kozlova, S.R.; Zolotar, A.N.; Shidlovskaya, E.V.; Usachev, E.V.; Pochtovyi, A.A.; Kustova, D.D.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Major role of S-glycoprotein in providing immunogenicity and protective immunity in mRNA lipid nanoparticle vaccines based on SARS-CoV-2 structural proteins. bioRxiv, 2024, preprint. doi: 10.1101/2023.12.30.573713
  2. Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
  3. Sakhabutdinova, A. R.; Chemeris, A. V.; Garafutdinov, R. R. Detection of Specific RNA Targets by Multimerization. Biochemistry (Moscow), 2023, 88(5), 679–686. doi: 10.1134/S0006297923050103
  4. Hildebrandt, R.P.; Moss, K.R.; Janusz-Kaminska, A.; Knudson, L.A.; Denes, L.T.; Saxena, T.; Boggupalli, D.P.; Li, Z.; Lin, K.; Bassell, G.J.; Wang, E.T. Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes. Nature Communications, 2023, 14, 3427. doi: 10.1038/s41467-023-38923-6
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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