Phosphate CPG 500 solid support
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13860 | 100 mg | $40 | in stock | |
| 43860 | 1 g | $170 | in stock |
Phosphate CPG is a solid support for the synthesis of 3'-phosphorylated oligonucleotides. The phosphate group can be used as a blocking functionality to prevent 3'-5' exonuclease activity of some polymerases.
Upon cleavage from the support, a phosphate group remains at the 3' end of the oligonucleotide. It does not require any changes in oligo synthesis and deprotection protocols.
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General properties
| Appearance: | off-white beads |
| Quality control: | NMR 1H, HPLC-MS (95%) of bound reagent, loading measurement, functional testing (oligo synthesis) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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