HMRhoNox-M, Fe(II)-selective fluorescent probe
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3317-50ug | 50 ug |
$159.00
|
in stock | |
| 3317-500ug | 500 ug |
$950.00
|
in stock |
HMRhoNox-M (also known as LysoRhoNox) is a Fe2+-selective fluorescent probe based on the N-oxide-controlled spirocyclization of tetramethyl-hydroxymethyl rhodamine.
In the absence of Fe2+, HMRhoNox-M exists in the non-fluorescent spirocyclic form showing only negligible fluorescence in an aqueous buffer and at physiological pH. The addition of Fe2+ induces a 60-fold increase of the fluorescence signal at 575 nm through the deoxygenation of the dialkylamino group and the transition of the probe to an open fluorescent form. HMRhoNox-M responds to Fe2+ in a dose-dependent manner.
The fluorescence response of HMRhoNox-M is highly selective for Fe2+ over other transition metal ions, including Fe3+, alkali metal ions, and alkaline earth metal ions.
HMRhoNox-M is the cell-permeant probe that is mainly localized in lysosomes. It is suitable for monitoring fluctuations of endogenous labile iron in living cells, including the transferrin-induced Fe uptake. Staining with HMRhoNox-M is incompatible with subsequent cell fixation.
Product in action
A549 cells stained with Fe(II)-selective fluorescent probe HMRhoNox-M (3317-x) and DAPI
Absorption and emission spectra of HMRhoNox-M
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General properties
| Appearance: | beige-pinkish crystals |
| Molecular weight: | 388.47 |
| Molecular formula: | C24H24N2O3 |
| Solubility: | DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| Emission maximum, nm: | 575 |
Product citations
- Litasova, E. V..; Matyushenko, V. A.; Sokolov, A. V.; Mezhenskaya, D. А.; Myznikov, L. V.; Sokolova, N. B.; Gorbunov, N. P.; Berson, Y. M.; Rak, A. Y.; Isakova-Sivak, I. N. Antiviral activity assessment of specific azo compounds against SARS-CoV-2 in in vitro experiment. Cytokines and inflammation, 2025, 22(3), 128–137. doi: 10.17816/CI695474
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