TAMRA-2,4-dinitroaniline (TMR-DN)

Cat. # Quantity Price Lead time
2641-1mg 1 mg $190 in stock
2641-5mg 5 mg $690 in stock
2641-10mg 10 mg $1000 in stock
2641-100mg 100 mg please inquire in stock
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TMR DN is a probe, the 5-carboxy tetramethylrhodamine (TMR) linked with a quencher moiety, dinitroaniline (DN), enabling contact quenching, non-toxic for cells, and capable cell membrane penetration.

The core of the molecule is represented by an aromatic fragment bearing a negatively charged carboxylic group, it possesses bright fluorescence properties, and at the same time, it reduces nonspecific binding to genomic DNA or RNA.

RNA-based sensors and TMR DN are used for intracellular visualization of mRNA and rRNA in live prokaryotic or eukaryotic cells. Tandem repeat aptamers (e.g., sulforhodamine-binding RNA aptamer (SRB-2)) may provide an advantage for the visualization of less stable RNA and those of low content quantities in cells. The advantage of the SRB-2/TMR DN system is its high brightness, comparable to GFP (green fluorescent protein), showing a smaller complex size. SRB-2 is also orthogonal to Spinach/Broccoli aptamers, and no crossreactivity between aptamers and ligands (TMR DN and DFHBI ((3,5-difluoro-4-hydroxybenzylidene)) occurs, thus TMR DN and SRB-2 can be used along with the other probe/aptamer pairs for visualization and dynamic imaging in parallel for several RNA [1]. The excitation and emission maxima of the complex SRB-2/TMR DN are 561 and 587 nm, respectively, lying in the orange region of the spectrum where the cellular auto-fluorescence is low [2].

[1] Rigumula Wu et al. Ratiometric Fluorogenic RNA-Based Sensors for Imaging Live-Cell Dynamics of Small Molecules. ACS Applied Bio Materials. 2020. 3(5). 2633-2642.

[2] Murat Sunbul & Andres Jäschke. SRB-2: a promiscuous rainbow aptamer for live-cell RNA imaging. Nucleic acids research. 2018. 46(18).

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General properties

Appearance: red crystals
Molecular weight: 726.73
Molecular formula: C37H38N6O10
IUPAC name: 3',6'-bis(dimethylamino)-N-(2-(2-(2-((2,4-dinitrophenyl)amino)ethoxy)ethoxy)ethyl)-3-oxo-3',9a'-dihydro-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxamide
Solubility: soluble in DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications
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