FAM-11-dCTP, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2713-50nmol | 50 nmol |
$115
|
in stock | |
| 2713-250nmol | 250 nmol |
$325
|
in stock | |
| 2713-500nmol | 500 nmol |
$500
|
in stock | |
| 2713-1umol | 1 umol |
$910
|
20 days |
6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).
FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.
FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation.
The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.
Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.
Absorption and emission spectra of FAM
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Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.General properties
| Appearance: | yellow to orange solid |
| Molecular weight: | 1012.5 |
| Molecular formula: | C39H40N5Li3O20P3 |
| IUPAC name: | ((2R,3S,5R)-5-(4-amino-5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | good in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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