Cyanine5 maleimide
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
13080 | 1 mg | $125 | in stock | |
23080 | 5 mg | $260 | in stock | |
43080 | 25 mg | $510 | in stock | |
53080 | 50 mg | $895 | in stock | |
63080 | 100 mg | $1490 | in stock |
Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.
Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.
For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.
Cyanine5 excitation and emission spectra
Recommended protocol
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sulfo-Cyanine7 NHS ester
sulfo-Cyanine7 NHS ester is a water-soluble, near-infrared, amine-reactive dye.TAMRA maleimide, 6-isomer
TAMRA is a well known xanthene dye of rhodamine series. This is a thiol reactive maleimide for the labeling of SH groups in proteins and peptides. Pure 6-isomer.FAM NHS ester, 6-isomer
Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.General properties
Appearance: | dark blue powder |
Molecular weight: | 605.8 |
CAS number: | 1437872-46-2 (without anion) |
Molecular formula: | C38H45N4O3 |
IUPAC name: | 3H-Indolium, 2-[5-[1-[6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-trimethyl- |
Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
Product citations
- Huang, L.; Liu, P.; Du, Y.; Pan, D.; Lee, A.; Wolfe, S.A.; Wang, Y.-X. A brown fat-enriched adipokine, ASRA, is a leptin receptor antagonist that stimulates appetite. bioRxiv, 2023, preprint. doi: 10.1101/2023.09.12.557454
- Chen, L.; Xu, N.; Wang, P.; Zhu, H.; Zhang, Z.; Yang, Z.; Zhang, W.; Guo, H.; Lin, J. Nanoalbumin–Prodrug Conjugates Prepared via a Thiolation-and-Conjugation Method Improve Cancer Chemotherapy and Immune Checkpoint Blockade Therapy by Promoting CD8+ T-Cell Infiltration. Bioengineering & Translational Medicine, 2023, 8(1), e10377. doi: 10.1002/btm2.10377
- Song, Q.-X.; Liu, N.-N.; Liu, Z.-X.; Zhang, Y.-Z.; Rety, S.; Hou, X.-M.; Xi, X.-G. Nonstructural N- and C-Tails of Dbp2 Confer the Protein Full Helicase Activities. Journal of Biological Chemistry, 2023, 299(5), 104592. doi: 10.1016/j.jbc.2023.104592
- Visentin, A.; Demitroff, N.; Salgueiro, M.; Borkosky, S. S.; Uversky, V. N.; Camporeale, G.; de Prat-Gay, G. Assembly of the Tripartite and RNA Condensates of the Respiratory Syncytial Virus Factory Proteins In Vitro: Role of the Transcription Antiterminator M2-1. Viruses, 2023, 15(6), 1329. doi: 10.3390/v15061329
Cy® is a trademark of GE Healthcare.
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