LumiCell CDr20 Microglia Stain

Neuroscience Flow cytometry Microscopy
Cat. # Quantity Price Lead time
3570-50ug 50 ug $50 in stock
3570-500ug 500 ug $350 in stock
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CDr20 (Cell Designation red 20) is a high-performance fluorogenic chemical probe for labeling microglia in both cell cultures and live brains. CDr20 is a substrate of microglia-specific UDP-glucuronosyltransferase Ugt1a7c. The glucuronidation of CDr20 by Ugt1a7c produces bright red fluorescence in microglial cells coinciding with the expression of markers P2ry12, Csf1r, Cx3cr1, and Iba-1 [1].

CDr20 could be a valuable tool for identifying and visualizing microglia in neural disorder studies both in vitro and in vivo and for CDr20-based fluorescent-activated microglial cell sorting (FACS).

[1] Kim B. et al. Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate. Angew. Chem. Int. Ed. Engl. 2019. 58(24). 7972-7976.

BV2 microglial cells stained with LumiCell CDr20 Microglia Stain (3570-x). BV2 microglial cells were stained with LumiCell CDr20 Microglia Stain (3570-x, 80 nM) for 30 min at 37°C, fixed with 2% paraformaldehyde, and covered with LumiMount DAPI Fluorescence Mounting Medium (x3144). The images were taken on a confocal microscope (excitation at 543 nm, emission at 650 nm).
LumiCell CDr20 Microglia Stain, 3570-500ug
LumiCell CDr20 Microglia Stain, 3570-500ug
LumiCell CDr20 Microglia Stain, 3570-500ug
Structure of CDr20
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Absorption and emission spectra of CDr20 Microglia Stain

Absorption and emission spectra of CDr20 Microglia Stain

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General properties

Appearance: greenish black crystals
Molecular weight: 354.17
CAS number: 1201643-01-7
Molecular formula: C19H17BF2N2O2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 562
ε, L⋅mol−1⋅cm−1: 97500
Emission maximum, nm: 581
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