TO-TAP-3, far-red fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A4610 50 uL, 5 mM/DMSO $85 in stock
24610 200 uL, 5 mM/DMSO $320 in stock
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TO-TAP-3 (Thiazole Red Monomer, also known as TO-PRO®-3) is a far-red fluorescent carbocyanine monomeric dye. TO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

TO-TAP-3 has a bright fluorescence signal and low background. The dye is useful for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in microscopy. It may be used for monitoring of cell viability and dead cell detection in culture. The long-wavelength fluorescence of TO-TAP-3 is well separated from green and red fluorophores, which makes it ideal for multicolor fluorescence labeling experiments.

Absorption and emission spectra of TO-TAP-3 (DNA–dye complex)

Absorption and emission spectra of TO-TAP-3 (DNA–dye complex)

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General properties

Appearance: blue solution
Molecular weight: 671.43
CAS number: 157199-63-8
Molecular formula: C26H31I2N3S
IUPAC name: trimethyl-[3-[4-[(E,3Z)-3-(3-methyl-1,3-benzothiazol-2-ylidene)prop-1-enyl]quinolin-1-ium-1-yl]propyl]azanium;diiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 643
Emission maximum, nm: 658
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