Biotin-11-dUTP
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1553-250nmol | 250 nmol | $140 | in stock | |
| 1553-500nmol | 500 nmol | $235 | in stock | |
| 1553-1umol | 1 umol | $360 | in stock | |
| 1553-5umol | 5 umol | please inquire | in stock |
Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation, polymerase chain reaction (PCR), random priming, and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.
«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.
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A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).General properties
| Appearance: | colorless solid |
| Molecular weight: | 878.54 |
| Molecular formula: | C28H40N6Li3O17P3S |
| IUPAC name: | ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Product citations
- Krasikova, A.; Kulikova, T.; Schelkunov, M.; Makarova, N.; Fedotova, A.; Berngardt, V.; Maslova, A.; Fedorov, A. The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.05.577752
- Krasikova, A.; Kulikova, T.; Rodriguez Ramos, J. S.; Maslova, A. Assignment of the Somatic A/B Compartments to Chromatin Domains in Giant Transcriptionally Active Lampbrush Chromosomes. Epigenetics & Chromatin, 2023, 16(1), 24. doi: 10.1186/s13072-023-00499-2
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