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ROS indicators
DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein)

DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein)

Microscopy Flow cytometry ROS sensing

Cat. #	Quantity	Price	Lead time	Buy this product
SB100	50 ug	$22	in stock (MOQ 10 pcs.)
1B100	1 mg	$365	21 days

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DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein) is a cell-impermeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM does not need to be activated by cytosolic enzymes and is suitable to detect NO in extracellular matrix.

The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .

The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.

DAF-FM should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.

The cell-permeant version of DAF FM — DAF-FM DA is also available.

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General properties

Appearance:	yellow to brown solid
Molecular weight:	412.35
CAS number:	254109-20-1
Molecular formula:	C₂₁H₁₄F₂N₂O₅
Solubility:	good in methanol, DMSO, DMF and water; limited in water; poor in acetonitrile and methylene chloride
Quality control:	NMR ¹H and HPLC-MS (90+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.