AF 568 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15880 | 1 mg |
$99.00
|
12 days | |
| 25880 | 5 mg |
$339.00
|
12 days | |
| 45880 | 25 mg |
$789.90
|
12 days | |
| 65880 | 100 mg |
$2199.00
|
12 days |
AF 568 maleimide is a bright orange-red fluorescent dye reactive toward thiol groups. The maleimide moiety selectively conjugates with sulfhydryl groups of cysteine residues in proteins, peptides, antibodies, and other biomolecules, forming stable thioether bonds. The dye provides high fluorescence intensity and excellent photostability, enabling efficient labeling for fluorescence microscopy, flow cytometry, and other bioanalytical applications.
AF 568 exhibits excitation and emission maxima compatible with standard TRITC/Cyanine3 imaging settings. The dye is well suited for multicolor experiments due to its minimal spectral overlap with green and far-red fluorophores.
Maleimide conjugation reactions proceed efficiently at near-neutral pH (typically pH 6.5–7.5), enabling selective labeling of free thiols under mild conditions. AF 568 maleimide can be used for labeling proteins, oligonucleotides, nanoparticles, and other thiol-containing molecules.
Absorption and emission spectra of AF 568
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General properties
| Appearance: | purple powder |
| Molecular weight: | 935.13 |
| Molecular formula: | C42H40K2N4O12S2 |
| Solubility: | water, DMSO |
| Quality control: | NMR 1H, HPLC-MS (90%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 572 |
| ε, L⋅mol−1⋅cm−1: | 94238 |
| Emission maximum, nm: | 598 |
| Fluorescence quantum yield: | 0.912 |
| CF260: | 0.4 |
| CF280: | 0.32 |
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