SynaptoProbe Green
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3762-100ug | 100 ug | – | in stock | |
| 3762-1mg | 1 mg | $132 | in stock | |
| 3762-5mg | 5 mg | $350 | in stock |
SynaptoProbe Green is an analog of FM®1-43, a widely used green fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.
This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.
SynaptoProbe Green can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.
Absorption and emission spectra of SynaptoProbe Green
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General properties
| Appearance: | light red solid |
| Molecular weight: | 705.55 |
| CAS number: | 149838-22-2 (dibromide) |
| Molecular formula: | C30H49I2N3 |
| IUPAC name: | N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Diiodide |
| Solubility: | good in water |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 55800 |
| Emission maximum, nm: | 594 |
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