SiRhius® 650 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11S30 | 1 mg |
$199.00
|
in stock | |
| 21S30 | 5 mg |
$499.00
|
in stock | |
| 51S30 | 50 mg |
please inquire
|
7 days |
SiRhius® 650 azide is a far-red fluorescent dye functionalized with an azide group for bioorthogonal labeling via click chemistry. The probe is designed for rapid and selective conjugation to alkyne-bearing biomolecules using copper-catalyzed (CuAAC) or strain-promoted (SPAAC) cycloaddition reactions.
The SiRhius® 650 fluorophore exhibits excitation and emission maxima in the far-red region (~650/670 nm), enabling deep tissue penetration and low background autofluorescence. Its high molar absorptivity and quantum yield provide a bright signal, while the photostable scaffold enables extended imaging applications, including live-cell and super-resolution microscopy.
The azide functionality is chemically stable under physiological conditions yet highly reactive in click reactions, allowing efficient labeling of proteins, nucleic acids, lipids, and other biomolecules modified with terminal alkynes. The relatively compact size of the azide group minimizes perturbation of the target molecule, preserving biological function.
SiRhius 650® azide is suitable for fluorescence microscopy, flow cytometry, and in vitro or in vivo imaging workflows where far-red emission and high specificity of labeling are required.
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General properties
| Appearance: | light-blue crystals |
| Molecular weight: | 554.71 |
| CAS number: | 3031722-00-3 |
| Molecular formula: | C30H34N6O3Si |
| Solubility: | DCM, acetonitrile, triethylamine, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 112000 |
| Emission maximum, nm: | 669 |
| Fluorescence quantum yield: | 0.57 |
| CF260: | 0.07 |
| CF280: | 0.02 |
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