FAM phosphoramidite, 6-isomer (hydroxyprolinol)

Cat. # Quantity Price Lead time
25360 250 mg $180 in stock
45360 1 g $575 in stock
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FAM phosphoramidite, 6-isomer (Pro) is a hydroxyprolinol-derived reagent suitable for 5’ and internal labeling. Hydroxyprolinol-based (FAM) phosphoramidite is a modifying reagent for the synthesis of labeled with fluorescein oligonucleotides.

For the purpose of the introduction of fluorescent labels this type of phosphoramidites is obtained with a 6-aminohexanoate linkage as a spacer between the skeleton and the functional group.

This modifying reagent with the core of hydroxyprolinol, also has a dimethoxythrityl protection group making available reversed phase HPLC (RP-HPLC) or reversed-phase cartridge purification.

Recommendations for using the reagent:

Coupling: 6 min.

Deprotection: Standard conditions with 25 % ammonium hydroxide; deprotection time depends on the composition of nucleic acids and their protective groups.

AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40 % aqueous methylamine) can be used for 2 hours at room temperature or 10 min at 65 °C.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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SIMA phosphoramidite, 6-isomer (hydroxyprolinol)

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General properties

Appearance: off-white solid
Molecular weight: 1259.44
Molecular formula: C72H83N4O14P
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
Emission maximum, nm: 517

Oligo synthesis details

Coupling conditions: 6 min coupling time recommended
Deprotection conditions: identical to protected nucleobases
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