BDP FL NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11420 | 1 mg | $125.00 | in stock | |
| 21420 | 5 mg | $260.00 | in stock | |
| 41420 | 25 mg |
$510.00
|
in stock | |
| 51420 | 50 mg |
$895.00
|
in stock | |
| 61420 | 100 mg |
$1490.00
|
in stock |
BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein. An amino-reactive dye for the labeling of proteins and peptides.
While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY™ FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.
The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.
The dye is a good replacement for fluorescein (FAM), DyLight™ 488, Cy2™, and other 488 nm dyes.
Absorption and emission spectra of BDP FL
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| Appearance: | orange solid |
| Mass spec M+ increment: | 274.1 |
| Molecular weight: | 389.16 |
| CAS number: | 146616-66-2 |
| Molecular formula: | C18H18BF2N3O4 |
| Solubility: | Good in organic solvents (DMF, DMSO, dichlromethane), limited in water |
| Quality control: | NMR 1H, HPLC-MS (95+%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 503 |
| ε, L⋅mol−1⋅cm−1: | 92000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
Product citations
- Ji, F.; Hur, M.; Hur, S.; Wang, S.; Sarkar, P.; Shao, S.; Aispuro, D.; Cong, X.; Hu, Y.; Li, Z.; Xue, M. Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage. ACS Bio Med Chem Au, 2023, 3(3), 283–294. doi: 10.1021/acsbiomedchemau.3c00018
- Santos-Coquillat, A.; González, M. I.; Clemente-Moragón, A.; González-Arjona, M.; Albaladejo-García, V.; Peinado, H.; Muñoz, J.; Ximénez Embún, P.; Ibañez, B.; Oliver, E.; Desco, M.; Salinas, B. Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging. Small, 2022, 18(6), 2105421. doi: 10.1002/smll.202105421
- Nascimento, A.; Pedro, M.N.S.; Pinto, I.F.; Aires-Barros, M.R.; Azevedo, A.M. Microfluidics as a high-throughput solution for chromatographic process development – The complexity of multimodal chromatography used as a proof of concept. Journal of Chromatography A, 2021, 1658, 462618. doi: 10.1016/j.chroma.2021.462618
- Best, Q.A.; Haack, R.A.; Swift, K.M.; Bax, B.M.; Tetin, S.Y.; Hershberger, S.J. A Rainbow of Acridinium Chemiluminescence. Luminescence, 2021, 36(4), 1097–1106. doi: 10.1002/bio.4038
BDP® is a trademark of Lumiprobe Corporation. BODIPY™, DyLight™ are registered trademarks of Thermo Fisher Scientific in some countries. Cy™ is a registered trademark of Cytiva in some countries.
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