BDP® FL ceramide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3057-50ug | 50 ug |
–
|
in stock | |
| 3057-250ug | 250 ug |
$149.00
|
in stock |
Ceramides are precursors of sphingolipids composed of sphingosine and a fatty acid joined by an amide bond. This BDP FL ceramide is a synthetic fluorescent lipid, a conjugate of green-emitting BDP FL fluorophore with sphingosine. Inside the cell, BDP FL ceramide is incorporated into the membranes of the Golgi apparatus, so this stain is widely used in cell biology to visualize the Golgi apparatus in living and fixed cells with fluorescence microscopy.
Product in action
Vital staining of the Golgi apparatus of human primary fibroblasts
Live CHO cells stained with the Golgi apparatus stain BDP FL ceramide and nuclear stain Hoechst 33342
Live RAW264.7 cells stained with the Golgi apparatus stain BDP FL ceramide and nuclear stain Hoechst 33342
Absorption and emission spectra of BDP FL
Recommended protocol
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General properties
| Appearance: | orange semisolid |
| Molecular weight: | 573.57 |
| CAS number: | 2730893-01-1 |
| Molecular formula: | C32H50BF2N3O3 |
| Solubility: | good in many organic solvents |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 503 |
| ε, L⋅mol−1⋅cm−1: | 85000 |
| Emission maximum, nm: | 509 |
| Fluorescence quantum yield: | 0.97 |
| CF260: | 0.015 |
| CF280: | 0.027 |
Product citations
- Bal, N. V.; Oblasov, I.; Ierusalimsky, V. N.; Shvadchenko, A. M.; Fortygina, P.; Idzhilova, O. S.; Borodinova, A. A.; Balaban, P. M.; Feofanov, A. V.; Nekrasova, O. V.; Nikitin, E. S. Potassium KCa3.1 Channel Overexpression Deteriorates Functionality and Availability of Channels at the Outer Cellular Membrane. Sci Rep, 2025, 15(1), 4928. doi: 10.1038/s41598-025-89097-8
BDP® is a trademark of Lumiprobe
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
Short link - lumiprobe.com/sh/p/8W
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