BDP 630/650 X NHS ester

NHS esters Fluorescence polarization Microscopy BDP 630/650 Protein labeling
Cat. # Quantity Price Lead time
15420 1 mg $110.00 in stock
25420 5 mg $210.00 in stock
45420 25 mg $410.00 in stock
55420 50 mg $695.00 in stock
65420 100 mg $1190.00 in stock
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BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.

This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.

BDP 630/650 X NHS ester, 25420
BDP 630/650 X NHS ester, 25420
BDP 630/650 X NHS ester, 25420
Structure of BDP 630/650 X NHS ester
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Absorption and emission spectra of BDP 630/650

Absorption and emission spectra of BDP 630/650

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General properties

Appearance: dark colored solid
Mass spec M+ increment: 545.2
Molecular weight: 660.5
CAS number: 2213445-35-1; 380367-48-6
Molecular formula: C33H31N4BF2O6S
IUPAC name: Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

Product citations

  1. Grätz, L.; Tropmann, K.; Bresinsky, M.; Müller, C.; Bernhardt, G.; Pockes, S. NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells. Scientific Reports, 2020, 10, 13288. doi: 10.1038/s41598-020-70332-3
  2. Gruber, C.G.; Pegoli, A.; Müller, C.; Grätz, L.; She, X.; Keller, M. Differently fluorescence-labelled dibenzodiazepinone-type muscarinic acetylcholine receptor ligands with high M2R affinity. RSC Medicinal Chemistry, 2020, 11(7), 823–832. doi: 10.1039/D0MD00137F
  3. Zhong, X.; Shtukenberg, A.G.; Liu, M.; Olson, I.A.; Weck, M.; Ward, M.D.; Kahr, B. Dislocation Generation by Microparticle Inclusions. Crystal Growth & Design, 2019, 19(11), 6649–6655. doi: 10.1021/acs.cgd.9b01041
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