BDP 630/650 X NHS ester
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15420
1 mg
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5 mg
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BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.
This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.
Absorption and emission spectra of BDP 630/650
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General properties
Appearance:
dark colored solid
Mass spec M+ increment:
545.2
Molecular weight:
660.5
CAS number:
2213445-35-1; 380367-48-6
Molecular formula:
C33 H31 N4 BF2 O6 S
IUPAC name:
Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate
Solubility:
good in DMF, DMSO
Quality control:
NMR 1 H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Product specifications
Spectral properties
Excitation/absorption maximum, nm:
628
ε, L⋅mol−1 ⋅cm−1 :
97000
Emission maximum, nm:
642
Fluorescence quantum yield:
0.91
CF260 :
0.029
CF280 :
0.035
Product citations
Grätz, L.; Tropmann, K.; Bresinsky, M.; Müller, C.; Bernhardt, G.; Pockes, S. NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells. Scientific Reports , 2020 , 10, 13288. doi: 10.1038/s41598-020-70332-3
Gruber, C.G.; Pegoli, A.; Müller, C.; Grätz, L.; She, X.; Keller, M. Differently fluorescence-labelled dibenzodiazepinone-type muscarinic acetylcholine receptor ligands with high M2 R affinity. RSC Medicinal Chemistry , 2020 , 11(7), 823–832. doi: 10.1039/D0MD00137F
Zhong, X.; Shtukenberg, A.G.; Liu, M.; Olson, I.A.; Weck, M.; Ward, M.D.; Kahr, B. Dislocation Generation by Microparticle Inclusions. Crystal Growth & Design , 2019 , 19(11), 6649–6655. doi: 10.1021/acs.cgd.9b01041
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