BDP 630/650 X NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $110.00 | in stock | |
| 25420 | 5 mg | $210.00 | in stock | |
| 45420 | 25 mg |
$410.00
|
in stock | |
| 55420 | 50 mg |
$695.00
|
in stock | |
| 65420 | 100 mg |
$1190.00
|
in stock |
BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.
This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.
Absorption and emission spectra of BDP 630/650
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Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 545.2 |
| Molecular weight: | 660.5 |
| CAS number: | 2213445-35-1; 380367-48-6 |
| Molecular formula: | C33H31N4BF2O6S |
| IUPAC name: | Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 628 |
| ε, L⋅mol−1⋅cm−1: | 97000 |
| Emission maximum, nm: | 642 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.029 |
| CF280: | 0.035 |
Product citations
- Grätz, L.; Tropmann, K.; Bresinsky, M.; Müller, C.; Bernhardt, G.; Pockes, S. NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells. Scientific Reports, 2020, 10, 13288. doi: 10.1038/s41598-020-70332-3
- Gruber, C.G.; Pegoli, A.; Müller, C.; Grätz, L.; She, X.; Keller, M. Differently fluorescence-labelled dibenzodiazepinone-type muscarinic acetylcholine receptor ligands with high M2R affinity. RSC Medicinal Chemistry, 2020, 11(7), 823–832. doi: 10.1039/D0MD00137F
- Zhong, X.; Shtukenberg, A.G.; Liu, M.; Olson, I.A.; Weck, M.; Ward, M.D.; Kahr, B. Dislocation Generation by Microparticle Inclusions. Crystal Growth & Design, 2019, 19(11), 6649–6655. doi: 10.1021/acs.cgd.9b01041
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