AF 594 C5 maleimide

AF 594 Maleimides

Cat. #	Quantity	Price	Lead time
17880	1 mg	$179.00	12 days
27880	5 mg	$599.00	12 days
47880	25 mg	$1680.00	12 days
67880	100 mg	$3049.00	12 days

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AF594 C5 Maleimide is a bright, photostable fluorescent dye functionalized with a maleimide group via a flexible pentamethylene (C5) spacer. The maleimide moiety reacts selectively and rapidly with sulfhydryl (—SH) groups at physiological pH (6.5–7.5), forming stable thioether conjugates. The extended hydrophilic spacer minimizes steric hindrance, thereby enhancing the labeling efficiency of proteins, antibodies, peptides, and other biomolecules containing free cysteine residues or thiols introduced through the reduction of disulfide bonds.

The dye exhibits excellent water solubility and low pH sensitivity. It is ideally suited for confocal microscopy, super-resolution microscopy (STED, SIM), and long-duration experiments due to its high photobleaching resistance.

Absorption and emission spectra of AF 594

Recommended protocol

Maleimide Labeling of Proteins and Other Thiolated Biomolecules

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General properties

Appearance:	dark blue crystals
Molecular weight:	887.00
Molecular formula:	C₄₄H₄₆N₄O₁₂S₂
IUPAC name:	4-[5-(2,5-dioxopyrrol-1-yl)pentylcarbamoyl]-2-[6,7,7,19,19,20-hexamethyl-9,17-bis(sulfomethyl)-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl]benzoic acid
Solubility:	good solubility in water, DMSO, DMF, and acetonitrile; limited solubility in DCM
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	586
ε, L⋅mol⁻¹⋅cm⁻¹:	105000
Emission maximum, nm:	613
Fluorescence quantum yield:	0.77
CF₂₆₀:	0.28
CF₂₈₀:	0.51

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.