ProteOrange is a fluorescent dye for the staining of proteins in gels. ProteOrange is an analog of SYPRO orange. It is more sensitive than Coumassie.
Sensitive fluorescence-based assay reagent 500x concentrate for quantitating purified protein samples. The reagent shows minimal variability for different proteins.
The core of this Protein Quantification Kit is a very sensitive ProteOrange fluorescent dye. This dye is suitable for use with spectrofluorometers and microplate readers. The kit is optimised for protein quantification, with detection level as low as 10 ng/mL of protein in final solution.
ProteOrange is a convenient tool for staining relatively heavy (over 6 kDa) proteins during SDS-PAGE. ProteOrange can subsitute Coomassie, its detection limit is one order of magnitude smaller. It is not as sensitive as silver, however staining procedure is rather simple. Stained proteins can be visualized at 312-365 nm. Suitable for Western Blotting, for Triton X-100 gel electrophoresis. Not for staining proteins on membrane.
Step-by-step instructions on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.
User manual for highly sensitive protein quantification by fluorescence-based assay using ProteOrange reagent
ProteOrange dye concentration in stock solution.
Short description of Protein staining experience with ProteOrange dye from Lumiprobe.
Collection of recommended protocols for the use with Lumiprobe reagents: labeling of biomolecules, working with fluorescent dyes etc.
A brief description of quality system at Lumiprobe
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