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Product
ProteOrange® is a fluorescent dye for staining proteins in gels. It is an analog of SYPRO orange and is more sensitive than Coumassie.
Cat. #
40210
Product
ProteOrange® is a fluorescent protein-sensitive dye used for quantitating purified protein samples. The dye shows minimal variability for different proteins. This reagent is a 500× concentrate of ProteOrange dye.
Cat. #
41210
Ready kit
The core of this Protein Quantification Kit is a very sensitive ProteOrange® fluorescent dye. This dye is suitable for use with spectrofluorometers and microplate readers. The kit is optimised for protein quantification, with detection level as low as 10 ng/mL of protein in final solution.
Cat. #
14102
Protocol
ProteOrange is a convenient tool for staining relatively heavy (over 6 kDa) proteins during SDS-PAGE. ProteOrange can subsitute Coomassie, its detection limit is one order of magnitude smaller. It is not as sensitive as silver, however staining procedure is rather simple. Stained proteins can be visualized at 312-365 nm. Suitable for Western Blotting, for Triton X-100 gel electrophoresis. Not for staining proteins on membrane.
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Help Step-by-step instructions on staining proteins with ProteOrange from Lumiprobe in polyacrylamide gels.
Manual
User manual for highly sensitive protein quantification by fluorescence-based assay using ProteOrange reagent
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Help ProteOrange dye concentration in stock solution.
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Help Short description of Protein staining experience with ProteOrange dye from Lumiprobe.
Web page
Collection of recommended protocols for the use with Lumiprobe reagents: labeling of biomolecules, working with fluorescent dyes etc.
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