ProteOrange® Protein Quantification Kit

Protein quantification Fluorometry

Cat. #	Quantity	Price	Lead time	Buy this product
A4102	100 uL dye	–	1 days
14102	1 mL dye	$415	1 days

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ProteOrange^® Kit with a fluorescent dye allows protein quantification with higher sensitivity than that of spectrophotometry and other conventional methods used for protein quantification (Lowry, Bradford, BCA).

ProteOrange^® reagent, the key kit ingredient, exhibits almost no fluorescence in the free form. In a solution it forms complexes with protein molecules and exhibits increased fluorescence upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm. The kit can be used with any fluorometer that has a suitable excitation source and detection channel. In general, the working range of measured protein concentration is limited by the equipment sensitivity. The recommended range of concentrations that can be measured is 10 ng/mL to 10 µg/mL for cuvette fluorometers and 100 ng/mL to 10 µg/mL for plate fluorometers.

The kit contains ProteOrange fluorescent dye, buffer for preparation of dye working solution, and protein concentration standard (BSA, 2 mg/mL). To quantify the test protein, prepare dye working solution and use it to dilute test samples and samples with known protein concentration to generate a calibration curve. Protein concentration standards are prepared using bovine serum albumin from the kit or (if available) experimental protein identical to that in the test samples. Incubate prepared samples for 10 min at 95 °C to denature proteins, allow them to cool down to room temperature and measure fluorescence intensity.

Depending on sample volume, the number of measurements varies from 200 (sample volume 2.5 mL and assay volume 2 mL for a standard fluorometric cuvette) to 2000 (sample volume 250 µL and assay volume 200 µL for a 96-well plate).

Ready-to-use kit manual

ProteOrange® Protein Quantification Kit manual

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Ready-to-use kit for simple, tolerant-to-contaminants protein quantification. The kit includes concentrated assay reagent, dilution buffer, and prediluted BSA standards.

ProteOrange® Protein Quantification Reagent, 500×

ProteOrange® is a fluorescent protein-sensitive dye used for quantitating purified protein samples. The dye shows minimal variability for different proteins. This reagent is a 500× concentrate of ProteOrange dye.

QuDye® Protein Quantification Reagent, 200×

Highly sensitive and selective reagent for fluorometric quantification of protein concentration, using both fluorescence microplate readers and cuvette-based devices. The dye is highly tolerant to non-protein impurities, except surface acting agents.

General properties

Storage conditions:	Store at 4 °C. Warm up to 20 °C before use. Transportation: at room temperature for up to 3 weeks.
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.