CM-DiI, lipophilic tracer

Neuroscience Cell proliferation Microscopy Flow cytometry Cell membrane
Cat. # Quantity Price Lead time
3513-50ug 50 ug –   in stock
3513-1mg 1 mg $349 in stock
Sample request
Found better price? Let us know and we will propose the way forward!

CM-DiI is an orange-red fluorescent carbocyanine dye containing thiol-reactive chloromethyl (CM) moiety. CM-DiI labels plasma membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes.

CM-DiI is used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. The dye is transferred to daughter cells and does not leak to adjacent cells in a population. CM-DiI staining displays bright fluorescence for at least 72 hours (around three to six cell generations). The dye shows little cytotoxicity inside the cell, with minimal effects on the proliferative ability or biology of the cell.

Unlike other membrane dyes, CM-DiI has increased water solubility and can be fixed using traditional aldehyde fixatives. CM-DiI also persists in cells after permeabilization procedures. These make it feasible to detect labeled cells in combination with other histological techniques such as immunochemistry, optical clearing, etc.

CM-DiI, lipophilic tracer, 3513-1mg
CM-DiI, lipophilic tracer, 3513-1mg
CM-DiI, lipophilic tracer, 3513-1mg
Structure of CM-DiI, lipophilic tracer
Download in
ChemDraw format

Absorption and emission spectra of CM-DiI

Absorption and emission spectra of CM-DiI

Customers also purchased with this product

LumiTracker® ER Green

Green fluorescent stain for selective labeling of endoplasmic reticulum (ER) in live cells.

BDP® 493/503 lipid stain

BDP 493/503 is a lipophilic green fluorescent dye for the staining and tracing of cell membranes, lipids, and oils.

LumiTracker® Mito Red CMXRos

Сell-permeant, cationic, red-fluorescent dye for staining active mitochondria in living cells in a potential-dependent manner. The stain is compatible with aldehyde fixation.

General properties

Appearance: red powder
Molecular weight: 1142.96
CAS number: 180854-97-1
Molecular formula: C68H105ClIN3O
Solubility: in DMSO, DMF, ethanol
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 556
ε, L⋅mol−1⋅cm−1: 129500
Emission maximum, nm: 571
Fluorescence quantum yield: 0.16
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.