BCECF AM, green fluorescent pH indicator

Microscopy

Cat. #	Quantity	Price	Lead time	Buy this product
3698-50ug	50 ug	$16	12 days (packed by 20 pcs.)
3698-1mg	1 mg	$169	7 days

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BCECF AM (2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester) is a cell-permeant, dual-excitation ratiometric fluorescent indicator for measuring changes in the intracellular pH.

Cytosolic esterases hydrolyze BCECF AM to yield BCECF, a polar fluorescein derivative held by cells. BCECF is stable in the cells and has an efflux half-life of over two hours. Intracellular pH is estimated by determining the pH-dependent ratio of emission intensity at 535 nm when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach can be carried out using spectrofluorometry or flow cytometry methods.

BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

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Product in action

Live culture of MDCK kidney epithelial cells stained with the fluorescent pH indicator BCECF AM

General properties

Appearance:	white powder
Molecular weight:	556.47
CAS number:	117464-70-7
Molecular formula:	C₃₀H₂₀O₁₁
IUPAC name:	3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester.
Solubility:	DMSO, ethyl acetate, toluene, acetonitrile
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.