AF 430 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10880 | 1 mg | $140 | in stock | |
| 20880 | 5 mg |
$450
|
in stock | |
| 40880 | 25 mg |
$1460
|
in stock | |
| 50880 | 50 mg |
$1890
|
in stock | |
| 60880 | 100 mg |
$2640
|
in stock |
AF 430 is a hydrophilic dye of a coumarin nature. The dye is used in flow cytometry.
This maleimide derivative is reactive against thiol groups. Therefore, it allows labeling of many proteins, including those residing on the cell surface.
Absorption and emission spectra of AF 430
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sulfo-Cyanine3 maleimide
Water soluble thiol-reactive derivative of sulfo-Cyanine3, a Cy3 analog.sulfo-Cyanine3.5 NHS ester
Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.Cyanine5 maleimide
Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.General properties
| Appearance: | yellow solid |
| Mass spec M+ increment: | 625.2 |
| Molecular weight: | 726.8 |
| Molecular formula: | C34H45N4F3O8S |
| IUPAC name: | (8-{6-[2-(2,5-Dioxo-1H-pyrrol-1-yl)ethylamino]-6-oxohexyl}-7,7-dimethyl-2-oxo-4-(trifluoromethyl)-1-oxa-8-aza-5,6,7,8-tetrahydroanthr-5-yl)methanesulfonic acid |
| Solubility: | soluble in water, polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
Product citations
- Yoo, W.; Song, Y.W.; Kim, J.; Ahn, J.; Kim, J.; Shin, Y.; Ryu, J.-K.; Kim, K.K. Molecular basis for SOX2-dependent regulation of super-enhancer activity. Nucleic Acids Research, 2023, 51(22), 11999-12019. doi: 10.1093/nar/gkad908
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