AF430 maleimide
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10880
1 mg
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20880
5 mg
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25 mg
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50880
50 mg
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60880
100 mg
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AF430 is a hydrophilic dye of coumarin nature, structurally identical to Alexa Fluor® 430. The dye is used in flow cytometry.
This maleimide derivative is reactive against thiol groups. Therefore, it allows to label many proteins, including those residing on cell surface.
Absorption and emission spectra of AF430
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General properties
Appearance:
yellow solid
Mass spec M+ increment:
625.2
Molecular weight:
726.8
Molecular formula:
C34 H45 N4 F3 O8 S
IUPAC name:
(8-{6-[2-(2,5-Dioxo-1H-pyrrol-1-yl)ethylamino]-6-oxohexyl}-7,7-dimethyl-2-oxo-4-(trifluoromethyl)-1-oxa-8-aza-5,6,7,8-tetrahydroanthr-5-yl)methanesulfonic acid
Solubility:
soluble in water, polar organic solvents (DMF, DMSO)
Quality control:
NMR 1 H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Product specifications
Spectral properties
Excitation maximum, nm:
430
ε, L⋅mol−1 ⋅cm−1 :
15955
Emission maximum, nm:
542
Fluorescence quantum yield:
0.23
CF260 :
0.06
CF280 :
0.06
Alexa Fluor™ is a trademark of Thermo Fisher Scientific
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