AF 430 maleimide

Cat. # Quantity Price Lead time
10880 1 mg $140 in stock
20880 5 mg $450 in stock
40880 25 mg $1460 in stock
50880 50 mg $1890 in stock
60880 100 mg $2640 in stock
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AF 430 is a hydrophilic dye of a coumarin nature. The dye is used in flow cytometry.

This maleimide derivative is reactive against thiol groups. Therefore, it allows labeling of many proteins, including those residing on the cell surface.

Absorption and emission spectra of AF 430

Absorption and emission spectra of AF 430

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General properties

Appearance: yellow solid
Mass spec M+ increment: 625.2
Molecular weight: 726.8
Molecular formula: C34H45N4F3O8S
IUPAC name: (8-{6-[2-(2,5-Dioxo-1H-pyrrol-1-yl)ethylamino]-6-oxohexyl}-7,7-dimethyl-2-oxo-4-(trifluoromethyl)-1-oxa-8-aza-5,6,7,8-tetrahydroanthr-5-yl)methanesulfonic acid
Solubility: soluble in water, polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23

Product citations

  1. Yoo, W.; Song, Y.W.; Kim, J.; Ahn, J.; Kim, J.; Shin, Y.; Ryu, J.-K.; Kim, K.K. Molecular basis for SOX2-dependent regulation of super-enhancer activity. Nucleic Acids Research, 2023, 51(22), 11999-12019. doi: 10.1093/nar/gkad908
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