QuDye® dsDNA BR Assay Kit

Fluorometry Nucleic acid quantification

Cat. #	Quantity	Price	Lead time
A9102	10 assays	–	1 days
19102	100 assays	$105 $67	1 days
1Z102 / includes tubes	100 assays	$125 $79	1 days
59102	500 assays	$290 $147	1 days
69102 / includes tubes	500 assays	$389 $176	1 days
89102	1000 assays	$499	1 days
79102 / aliquoted standard	1000 assays	$499	1 days

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This kit is intended for quantification of double-stranded DNA in the broad range with fluorometer (any version can be used). The kit includes QuDye dsDNA BR fluorescent dye concentrate 200×, buffer for preparation of dye working solution, and DNA concentration standards (0 and 100 ng/µL). QuDye dsDNA BR Reagent is selective to double-stranded DNA, so measurement results are not affected by RNA, free nucleotides, or protein contaminants. Other minor contaminants such as salts, detergents, and solvents have a non-significant effect on measurements results. This kit allows accurate dsDNA quantification in the range from 100 pg/µL to 1000 ng/µL for initial sample (DNA amount range for measuring: 2–1000 ng of DNA in 200 µL of the test sample).

The main kit component is QuDye dsDNA BR fluorescent dye, which selectively interacts with DNA and exhibits enhanced fluorescence upon binding to DNA molecule. This kit allows convenient dsDNA quantification in the broad concentration range and does not require a large volume of the initial sample (1–20 µL is required for the assay). All measurements are performed at room temperature and take on average 15–20 minutes (depending on the sample amount). Sample fluorescence is stable for 3 hours. To perform a measurement, prepare dye working solution, dilute DNA standards and experimental samples with it in individual tubes and perform fluorescence measurements using fluorometer. For your convenience, we offer kits including thin-wall tubes compatible with fluorometer.

This kit can be also used with QuDye Protein Quantification Kit to determine protein contaminants in the DNA sample.

For quantification of dsDNA of lower concentrations, use QuDye dsDNA HS Assay Kit with the range of initial sample concentrations of 10 pg/µL to 100 ng/µL.

Ready-to-use kit manual

QuDye® dsDNA BR Assay Kit manual

Calculator

Solution calculator

General properties

Storage conditions:	Store at 4 °С. Warm up to room temperature before use. Transportation: at room temperature for up to 3 weeks.
MSDS:	Download

Kit

Storage time in months:

References

Taira, Y.; Shimoji, Y.; Nakasone, T.; Arakaki, Y.; Nakamoto, T.; Kinjo, T.; Kudaka, W.; Mekaru, K.; Aoki, Y. A High-Risk Gestational Trophoblastic Neoplasia Derived from a Complete Hydatidiform Mole with Coexisting Fetus Identified by Short Tandem Repeats Analysis: A Case Report. Case Reports in Women’s Health, 2021, 31, e00336. doi: 10.1016/j.crwh.2021.e00336

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.