Nucleic acid quantification

Lumiprobe offers a selection of kits for quantifying biomolecules: nucleic acids. The measurement is based on the selective binding of dyes with the target molecules, and corresponding fluorescent response.

The measurement results correlate well with other measurement methods. The measurements are reproducible. The method has a wide dynamic range up to three orders of magnitude.

There are kits specifically designed for common fluorometers and any fluorescent reader with green (fluorescein or FAM, 490/520 nm) channel can be used for the measurements with proper calibration - all calculations are done intuitively using our DNA/Protein quantification calculator tool.

PreciseGreen dsDNA Quantification Kit

A kit for the quantification of dsDNA using PreciseGreen fluorescent dye.
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Cat. # Quantity Price Lead time
1102-20
20 assays
–   1 days
1102-200
/ 1 mL dye
200 assays
$450 1 days
B1102
/ 10 x 100 uL dye
200 assays
$500 1 days
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QuDye dsDNA BR Assay Kit

Ready kit for accurate DNA quantification with fluorometer in the broad range from 100 pg/µL to 1000 ng/µL. Common contaminants are well tolerated in the assay, only 1-20 µL of experimental sample is required. Measurements are performed at room temperature.
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Cat. # Quantity Price Lead time
A9102
10 assays
–   1 days
19102
100 assays
$105 1 days
1Z102
/ includes tubes
100 assays
$125 1 days
59102
500 assays
$290 1 days
69102
/ includes tubes
500 assays
$389 1 days
79102
/ aliquoted standard
1000 assays
$499 1 days
89102
1000 assays
$499 1 days
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QuDye dsDNA HS Assay Kit

Ready kit optimised for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL
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Cat. # Quantity Price Lead time
A2102
10 assays
–   1 days
12102
100 assays
$105 1 days
13102
/ includes tubes
100 assays
$125 1 days
52102
500 assays
$290 1 days
53102
/ includes tubes
500 assays
$389 1 days
72102
1000 assays
$499 1 days
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QuDye ssDNA Assay Kit

Ready kit for accurate and sensitive quantification of single-stranded DNA with fluorometer; the measurement range is from 50 pg/μL to 200 ng/μL for initial ssDNA sample.
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Cat. # Quantity Price Lead time
A7102
10 assays
–   1 days
17102
100 assays
$110 1 days
18102
/ includes tubes
100 assays
$130 1 days
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Ribo488 RNA assay kit compatible with plate readers

Ribo488 fluorescent dye kit for quantitative measurement of RNA concentration in solution. It is compatible with cuvette and microvolume fluorometers. Kit includes buffer and RNA standard.
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Cat. # Quantity Price Lead time
11520
/ standard 0 is to be made of buffer
200 assays
$125 1 days
21520
/ standard 0 is to be made of buffer
2000 assays
$950 1 days
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PreciseGreen dsDNA Quantification Reagent, 200×

PreciseGreen is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.
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Cat. # Quantity Price Lead time
12010 100 uL –   in stock
42010 1 mL $360 in stock
62010 25 mL please inquire in stock
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QuDye dsDNA BR Reagent, 200×

Concentrated reagent for quantitation of double-stranded DNA in a broad range initial sample concentration using a fluorescence plate reader or fluorometer.
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Cat. # Quantity Price Lead time
28010 30 uL –   in stock
68010 1.25 mL $207 in stock
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QuDye dsDNA HS Reagent, 200×

Concentrated reagent for high sensitive (HS) double-stranded (dsDNA) quantification to be used in any fluorescence reader.
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Cat. # Quantity Price Lead time
A3010 30 uL –   in stock
63010 1.25 mL $207 in stock
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Ribo488 RNA Quantification Reagent

Ribo488 is the fluorescent intercalating dye for quantitation of RNA.
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Cat. # Quantity Price Lead time
11510 100 uL –   in stock
41510 1 mL $850 in stock
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